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  • KD/KO Validated

PRDX3 Polyclonal antibody

PRDX3 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 10664-1-AP

Synonyms

peroxiredoxin 3, MER5, EC:1.11.1.24, AOP-1, AOP1



Tested Applications

Positive WB detected inHeLa cells, HEK-293 cells, MCF-7 cells, LNCaP cells, mouse brain tissue, mouse heart tissue
Positive IHC detected inhuman lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:16000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

10664-1-AP targets PRDX3 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PRDX3 fusion protein Ag1062
Full Name peroxiredoxin 3
Calculated Molecular Weight 27 kDa
Observed Molecular Weight 26-28 kDa
GenBank Accession NumberBC007062
Gene Symbol PRDX3
Gene ID (NCBI) 10935
RRIDAB_2284207
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PRDX3(Peroxiredoxin 3), also named as AOP1, HBC189 and MER5, belongs to the ahpC/TSA family. It is involved in redox regulation of the cell and protects radical-sensitive enzymes from oxidative damage by a radical-generating system. PRDX3 is required for MYC-mediated proliferation, transformation, and apoptosis after glucose withdrawal and essential for maintaining mitochondrial mass and membrane potential in transformed rat and human cells (PMID:12011429). PRDX3 acts synergistically with MAP3K13 to regulate the activation of NF-kappa-B in the cytosol.

Protocols

Product Specific Protocols
WB protocol for PRDX3 antibody 10664-1-APDownload protocol
IHC protocol for PRDX3 antibody 10664-1-APDownload protocol
IF protocol for PRDX3 antibody 10664-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Redox Biol

PMID: 32604037

Authors - Anindya Roy Chowdhury
humanWB,IHC

Redox Biol

LRPPRC regulates redox homeostasis via the circANKHD1/FOXM1 axis to enhance bladder urothelial carcinoma tumorigenesis.

Authors - Wen-Su Wei
humanWB

Redox Biol

Icariin alleviates uveitis by targeting peroxiredoxin 3 to modulate retinal microglia M1/M2 phenotypic polarization.

Authors - Guoqing Wang
  • KD Validated
ratIF

Oxid Med Cell Longev

Paeoniflorin Upregulates Mitochondrial Thioredoxin of Schwann Cells to Improve Diabetic Peripheral Neuropathy Indicated by 4D Label-Free Quantitative Proteomics.

Authors - Xinwei Yang
humanWB

Mol Cell Proteomics

Proteomics characterization of the cytotoxicity mechanism of ganoderic acid D and computer-automated estimation of the possible drug target network.

Authors - Yue Qing-Xi QX

J Clin Endocrinol Metab

Thioredoxin reductase 2 (TXNRD2) mutation associated with familial glucocorticoid deficiency (FGD).

Authors - Rathi Prasad

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.


FH

Guillermo (Verified Customer) (09-03-2022)

I trust in Proteintech, my favourite antibody company. 5/5 product.

  • Applications: Western Blot