PR3/PRTN3 Monoclonal antibody

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  WB analysis of human placenta using 67030-1-Ig

  human placenta tissue were subjected to SDS PAGE followed by western blot with 67030-1-Ig (PRTN3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IHC staining of human spleen using 67030-1-Ig

  Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 67030-1-Ig (PRTN3 antibody) at dilution of 1:2000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human spleen using 67030-1-Ig

  Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 67030-1-Ig (PRTN3 antibody) at dilution of 1:2000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human tonsillitis using 67030-1-Ig

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 67030-1-Ig (PRTN3 antibody) at dilution of 1:2000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human tonsillitis using 67030-1-Ig

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 67030-1-Ig (PRTN3 antibody) at dilution of 1:2000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of human tonsillitis using 67030-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using PRTN3 antibody (67030-1-Ig, Clone: 2D1B8 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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  IF Staining of human tonsillitis using 67030-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human tonsillitis tissue using PRTN3 antibody (67030-1-Ig, Clone: 2D1B8 ) at dilution of 1:400 and CoraLite®594-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-3). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

PR3/PRTN3 Monoclonal Antibody for WB, IHC, IF-P, ELISA

Cat No. 67030-1-Ig

Clone No.2D1B8

Host / Isotype

Mouse / IgG2b

Reactivity

human

Applications

WB, IHC, IF-P, ELISA

Synonyms

PRTN3, NP-4, Myeloblastin, MBN, EC:3.4.21.76

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Tested Applications

• Positive WB detected in: human placenta tissue
• Positive IHC detected in: human spleen tissue, human tonsillitis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF-P detected in: human tonsillitis tissue

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochemistry (IHC): IHC : 1:1000-1:4000
• Immunofluorescence (IF)-P: IF-P : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

67030-1-Ig targets PR3/PRTN3 in WB, IHC, IF-P, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen: PR3/PRTN3 fusion protein Ag25915
• Full Name: proteinase 3
• Calculated Molecular Weight: 256 aa, 28 kDa
• Observed Molecular Weight: 28 kDa
• GenBank Accession Number: BC096183
• Gene Symbol: PRTN3
• Gene ID (NCBI): 5657
• RRID: AB_2882345
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P24158
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.