PRR5 Polyclonal antibody

PRR5 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

1

Reviews (1)

Cat no : 17948-1-AP

Synonyms

Proline-rich protein 5, Proline rich protein 5, proline rich 5 (renal), PP610, FLJ20185k

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Validation Data Gallery

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  WB analysis of human kidney using 17948-1-AP

  human kidney tissue were subjected to SDS PAGE followed by western blot with 17948-1-AP (PRR5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 17948-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 17948-1-AP (PRR5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 17948-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 17948-1-AP (PRR5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of COLO 320 using 17948-1-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 17948-1-AP (PRR5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 17948-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 17948-1-AP (PRR5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 17948-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 17948-1-AP (PRR5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse brain using 17948-1-AP

  IP result of anti-PRR5 (IP:17948-1-AP, 3ug; Detection:17948-1-AP 1:300) with mouse brain tissue lysate 4000ug.

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  IHC staining of mouse testis using 17948-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 17948-1-AP (PRR5 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse testis using 17948-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 17948-1-AP (PRR5 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human placenta using 17948-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 17948-1-AP (PRR5 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human placenta using 17948-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 17948-1-AP (PRR5 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 17948-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 17948-1-AP (PRR5 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 17948-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 17948-1-AP (PRR5 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human placenta using 17948-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 17948-1-AP (PRR5 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human placenta using 17948-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 17948-1-AP (PRR5 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 17948-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 17948-1-AP (PRR5 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 17948-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 17948-1-AP (PRR5 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse kidney using 17948-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 17948-1-AP (PRR5 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse kidney using 17948-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 17948-1-AP (PRR5 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human kidney using 17948-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 17948-1-AP (PRR5 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human kidney using 17948-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 17948-1-AP (PRR5 antibody) at dilution of 1:100 (under 40x lens).

• 

  IF Staining of HepG2 using 17948-1-AP

  Immunofluorescent analysis of HepG2 cells, using PRR5 antibody 17948-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Tested Applications

• Positive WB detected in: human kidney tissue, COLO 320 cells, HEK-293 cells, HepG2 cells, human brain tissue, mouse brain tissue
• Positive IP detected in: mouse brain tissue
• Positive IHC detected in: mouse testis tissue, mouse brain tissue, mouse kidney tissue, human kidney tissue, human placenta tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2400
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• IHC: See 1 publications below

Product Information

17948-1-AP targets PRR5 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PRR5 fusion protein Ag12424
• Full Name: proline rich 5 (renal)
• Calculated Molecular Weight: 31 kDa, 43 kDa
• Observed Molecular Weight: 33 kDa, 50 kDa
• GenBank Accession Number: BC016921
• Gene Symbol: PRR5
• Gene ID (NCBI): 55615
• RRID: AB_2268865
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PRR5, also named as PROTOR1 and PP610, is a subunit of mTORC2, which regulates cell growth and survival in response to hormonal signals. PRR5 plays an important role in regulation of PDGFRB expression and in modulation of platelet-derived growth factor signaling. It acts as a tumor suppressor in breast cancer. PRR5 has some isoforms with MW 31-33 kDa and 41-50 kDa.

Publications

Species	Application	Title
human	IHC	Nat Cell Biol; m6A mRNA methylation regulates AKT activity to promote the proliferation and tumorigenicity of endometrial cancer.; Authors - Jun Liu

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Tominica (Verified Customer) (08-19-2019)

Rabbit polyclonal anti-FUS/TLS Ab-TLS-M generated against amino acids 260–274 of mouse FUS/TLS, a stretch where the sequence is identical to the human FUS/TLS sequence. works very well.

• Applications: Western Blot, Immunofluorescence,
• Cell Tissue Type: human motor neuron