PRPSAP1 Polyclonal antibody

PRPSAP1 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 16790-1-AP

Synonyms

PAP39, PRPSAP1

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Validation Data Gallery

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  WB analysis of human testis using 16790-1-AP

  human testis tissue were subjected to SDS PAGE followed by western blot with 16790-1-AP (PRPSAP1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse testis using 16790-1-AP

  IP result of anti-PRPSAP1 (IP:16790-1-AP, 4ug; Detection:16790-1-AP 1:300) with mouse testis tissue lysate 4000ug.

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  IHC staining of human cervical cancer using 16790-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 16790-1-AP (PRPSAP1 antibody) at dilution of 1:100 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human cervical cancer using 16790-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 16790-1-AP (PRPSAP1 antibody) at dilution of 1:100 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: human testis tissue
• Positive IP detected in: mouse testis tissue
• Positive IHC detected in: human liver cancer tissue, human cervical cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

16790-1-AP targets PRPSAP1 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PRPSAP1 fusion protein Ag10227
• Full Name: phosphoribosyl pyrophosphate synthetase-associated protein 1
• Calculated Molecular Weight: 356 aa, 39 kDa
• GenBank Accession Number: BC009012
• Gene Symbol: PRPSAP1
• Gene ID (NCBI): 5635
• RRID: AB_2171534
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.