PRPH2 Recombinant antibody, PBS Only

PRPH2 Uni-rAb^TM Recombinant Antibody for WB, IHC, IF-P, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF-P, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

242170F4

Cat no : 85043-1-PBS

Synonyms

Peripherin-2, Peripherin2, Peripherin 2, AVMD, AOFMD

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Validation Data Gallery

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  WB analysis using 85043-1-RR (same clone as 85043-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 85043-1-RR (PRPH2 antibody) at dilution of 1:12000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85043-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse eye using 85043-1-RR (same clone as 85043-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse eye tissue slide using 85043-1-RR (PRPH2 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85043-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse eye using 85043-1-RR (same clone as 85043-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse eye tissue slide using 85043-1-RR (PRPH2 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85043-1-PBS in a different storage buffer formulation.

• 

  IF Staining of mouse eye using 85043-1-RR (same clone as 85043-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse eye tissue using PRPH2 antibody (85043-1-RR, Clone: 242170F4 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85043-1-PBS in a different storage buffer formulation.

• 

  IF Staining of mouse eye using 85043-1-RR (same clone as 85043-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse eye tissue using PRPH2 antibody (85043-1-RR, Clone: 242170F4 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85043-1-PBS in a different storage buffer formulation.

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Product Information

85043-1-PBS targets PRPH2 in WB, IHC, IF-P, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: PRPH2 fusion protein Ag12555
• Full Name: peripherin 2 (retinal degeneration, slow)
• Calculated Molecular Weight: 346 aa, 39 kDa
• Observed Molecular Weight: 30-40 kDa
• GenBank Accession Number: BC074720
• Gene Symbol: PRPH2
• Gene ID (NCBI): 5961
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

Peripherin-2 (PRPH2), also known as retinal degeneration slow protein (RDS), is a photoreceptor-specific tetraspanin protein implicated in outer segment disk morphogenesis. It may function as an adhesion molecule involved in the stabilization and compaction of outer segment disks or in the maintenance of the curvature of the rim. Mutations in peripherin-2 are responsible for various retinal degenerative diseases including autosomal dominant retinitis pigmentosa (ADRP).