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PRPH2 Recombinant antibody

PRPH2 Uni-rAbTM Recombinant Antibody for WB, IHC, IF-P, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF-P, ELISA

Conjugate

Unconjugated

CloneNo.

242170F4

Cat no : 85043-1-RR

Synonyms

Peripherin-2, Peripherin2, Peripherin 2, AVMD, AOFMD

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using PRPH2 Recombinant antibody (85043-1-RR)

    WB analysis using 85043-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 85043-1-RR (PRPH2 antibody) at dilution of 1:12000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse eye tissue using PRPH2 Recombinant antibody (85043-1-RR)

    IHC staining of mouse eye using 85043-1-RR

    Immunohistochemical analysis of paraffin-embedded mouse eye tissue slide using 85043-1-RR (PRPH2 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse eye tissue using PRPH2 Recombinant antibody (85043-1-RR)

    IHC staining of mouse eye using 85043-1-RR

    Immunohistochemical analysis of paraffin-embedded mouse eye tissue slide using 85043-1-RR (PRPH2 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of mouse eye tissue using PRPH2 Recombinant antibody (85043-1-RR)

    IF Staining of mouse eye using 85043-1-RR

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse eye tissue using PRPH2 antibody (85043-1-RR, Clone: 242170F4 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of mouse eye tissue using PRPH2 Recombinant antibody (85043-1-RR)

    IF Staining of mouse eye using 85043-1-RR

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse eye tissue using PRPH2 antibody (85043-1-RR, Clone: 242170F4 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inmouse eye tissue, rat eye tissue
Positive IHC detected inmouse eye tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse eye tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:200-1:800
Immunofluorescence (IF)-PIF-P : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

85043-1-RR targets PRPH2 in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen PRPH2 fusion protein Ag12555
Full Name peripherin 2 (retinal degeneration, slow)
Calculated Molecular Weight 346 aa, 39 kDa
Observed Molecular Weight30-40 kDa
GenBank Accession NumberBC074720
Gene Symbol PRPH2
Gene ID (NCBI) 5961
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Peripherin-2 (PRPH2), also known as retinal degeneration slow protein (RDS), is a photoreceptor-specific tetraspanin protein implicated in outer segment disk morphogenesis. It may function as an adhesion molecule involved in the stabilization and compaction of outer segment disks or in the maintenance of the curvature of the rim. Mutations in peripherin-2 are responsible for various retinal degenerative diseases including autosomal dominant retinitis pigmentosa (ADRP).

Protocols

Product Specific Protocols
WB protocol for PRPH2 antibody 85043-1-RRDownload protocol
IHC protocol for PRPH2 antibody 85043-1-RRDownload protocol
IF protocol for PRPH2 antibody 85043-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols