PRPH2 Monoclonal antibody

PRPH2 Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse, rat, rabbit

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

2F11A1

Cat no : 68780-1-Ig

Synonyms

Peripherin-2, Peripherin2, Peripherin 2, AVMD, AOFMD

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Validation Data Gallery

• 

  WB analysis using 68780-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68780-1-Ig (PRPH2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 68780-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68780-1-Ig (PRPH2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: ARPE-19 cells, rabbit eye tissue, hTERT-RPE1 cells, rat eye tissue, mouse eye tissue, rabbit retina tissue, rat retina tissue, mouse retina tissue

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

68780-1-Ig targets PRPH2 in WB, ELISA applications and shows reactivity with human, mouse, rat, rabbit samples.

• Tested Reactivity: human, mouse, rat, rabbit
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: PRPH2 fusion protein Ag12555
• Full Name: peripherin 2 (retinal degeneration, slow)
• Calculated Molecular Weight: 346 aa, 39 kDa
• Observed Molecular Weight: 35-39 kDa
• GenBank Accession Number: BC074720
• Gene Symbol: PRPH2
• Gene ID (NCBI): 5961
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Peripherin-2 (PRPH2), also known as retinal degeneration slow protein (RDS), is a photoreceptor-specific tetraspanin protein implicated in outer segment disk morphogenesis. It may function as an adhesion molecule involved in stabilization and compaction of outer segment disks or in the maintenance of the curvature of the rim. Mutations in peripherin-2 are responsible for various retinal degenerative diseases including autosomal dominant retinitis pigmentosa (ADRP).