Peripherin Polyclonal antibody

Peripherin Polyclonal Antibody for WB, IHC, IF-P, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF-P, FC (Intra), ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 17399-1-AP

Synonyms

peripherin, PRPH, NEF4

---

Validation Data Gallery

• 

  WB analysis using 17399-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17399-1-AP (Peripherin antibody) at dilution of 1:120000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse colon using 17399-1-AP

  mouse colon tissue were subjected to SDS PAGE followed by western blot with 17399-1-AP (PRPH antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of COLO 320 using 17399-1-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 17399-1-AP (Peripherin Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human colon using 17399-1-AP

  human colon tissue were subjected to SDS PAGE followed by western blot with 17399-1-AP (PRPH antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of mouse brain using 17399-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 17399-1-AP (Peripherin antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon using 17399-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 17399-1-AP (Peripherin Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human colon using 17399-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 17399-1-AP (Peripherin Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of rat brain using 17399-1-AP

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 17399-1-AP (Peripherin antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human brain using 17399-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 17399-1-AP (PRPH antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human brain using 17399-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 17399-1-AP (PRPH antibody) at dilution of 1:100 (under 40x lens).

• 

  IF Staining of human colon using 17399-1-AP

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human colon tissue using Peripherin antibody (17399-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of human colon using 17399-1-AP

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human colon tissue using Peripherin antibody (17399-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  FC experiment of PC-12 using 17399-1-AP

  1X10^6 PC-12 cells were intracellularly stained with 0.4 ug Anti-Human Peripherin (17399-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

---

Tested Applications

• Positive WB detected in: mouse colon tissue, COLO 320 cells, human colon tissue, rat colon tissue, rat spinal cord tissue
• Positive IHC detected in: mouse brain tissue, human colon tissue, human brain tissue, rat brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF-P detected in: human colon tissue
• Positive FC (Intra) detected in: PC-12 cells
• Positive FC detected in: PC-12 cells

Recommended dilution

• Western Blot (WB): WB : 1:20000-1:100000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
• Flow Cytometry (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• IF: See 1 publications below

Product Information

17399-1-AP targets Peripherin in WB, IHC, IF-P, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peripherin fusion protein Ag11365
• Full Name: peripherin
• Calculated Molecular Weight: 470 aa, 54 kDa
• Observed Molecular Weight: 54-60 kDa
• GenBank Accession Number: BC032703
• Gene Symbol: Peripherin
• Gene ID (NCBI): 5630
• RRID: AB_2171195
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Peripherin is a neuron-specific intermediate filament protein that is particularly expressed in peripheral nervous system. It is used to study peripheral nerve development and regeneration, as it is highly expressed during elongation of axon. Peripherin is a routine marker for unmyelinated neurons in trigeminal ganglion (TG) and the dorsal root ganglion (DRG). Peripherin is also expressed in peri-insular areas of islets and it has been identified as a key autoantigen for type 1A diabetes.

Publications

Species	Application	Title
rat	IF	Biotechniques; Antibody incubation at 37°C improves fluorescent immunolabeling in free-floating thick tissue sections.; Authors - Xia Xiao