PRPF40B Polyclonal antibody

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  WB analysis of Y79 using 16929-1-AP

  WB result of PRPF40B antibody (16929-1-AP; 1:6000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PRPF40B transfected Y79 cells.

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  WB analysis using 16929-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16929-1-AP (PRPF40B antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Y79 using 16929-1-AP

  Y79 cells were subjected to SDS PAGE followed by western blot with 16929-1-AP (PRPF40B antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Y79 using 16929-1-AP

  Y79 cells were subjected to SDS PAGE followed by western blot with 16929-1-AP (PRPF40B antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of Y79 using 16929-1-AP

  IP result of anti-PRPF40B (IP:16929-1-AP, 4ug; Detection:16929-1-AP 1:5000) with Y79 cells lysate 2800 ug.

• Featured Product
• KD/KO Validated

PRPF40B Polyclonal Antibody for WB, IP, ELISA

Cat No. 16929-1-AP

Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF, IP, ELISA

Synonyms

Huntingtin yeast partner C, HYPC, PRPF40B

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Tested Applications

• Positive WB detected in: Y79 cells, K-562 cells, mouse testis tissue
• Positive IP detected in: Y79 cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:12000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• IF: See 1 publications below

Product Information

16929-1-AP targets PRPF40B in WB, IF, IP, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PRPF40B fusion protein Ag10361
• Full Name: PRP40 pre-mRNA processing factor 40 homolog B (S. cerevisiae)
• Calculated Molecular Weight: 871 aa, 99 kDa
• Observed Molecular Weight: 130-150 kDa
• GenBank Accession Number: BC067364
• Gene Symbol: PRPF40B
• Gene ID (NCBI): 25766
• RRID: AB_2171041
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q6NWY9
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	IF	Nucleic Acids Res; PRPF40A induces inclusion of exons in GC-rich regions important for human myeloid cell differentiation; Authors - Cheryl Weiqi Tan