PRPF3 Polyclonal antibody

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  WB analysis of Y79 using 10106-1-AP

  Y79 cells were subjected to SDS PAGE followed by western blot with 10106-1-AP (PRPF3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 10106-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 10106-1-AP (PRPF3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 10106-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 10106-1-AP (PRPF3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of L02 using 10106-1-AP

  L02 cells were subjected to SDS PAGE followed by western blot with 10106-1-AP (PRPF3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse liver using 10106-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 10106-1-AP (PRPF3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse liver using 10106-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 10106-1-AP (PRPF3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse liver using 10106-1-AP

  IP result of anti-PRPF3 (IP:10106-1-AP, 5ug; Detection:10106-1-AP 1:500) with mouse liver tissue lysate 4000ug.

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  IHC staining of human stomach using 10106-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 10106-1-AP (PRPF3 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

PRPF3 Polyclonal Antibody for WB, IHC, IP, ELISA

Cat No. 10106-1-AP

Publications(5)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, IP, ELISA

Synonyms

RP18, Prp3p, PRP3, Pre-mRNA-splicing factor 3, HPRP3P

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Tested Applications

• Positive WB detected in: Y79 cells, HepG2 cells, L02 cells, mouse brain tissue, mouse liver tissue
• Positive IP detected in: mouse liver tissue
• Positive IHC detected in: human stomach tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 4 publications below
• IF: See 1 publications below

Product Information

10106-1-AP targets PRPF3 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PRPF3 fusion protein Ag0154
• Full Name: PRP3 pre-mRNA processing factor 3 homolog (S. cerevisiae)
• Calculated Molecular Weight: 78 kDa
• Observed Molecular Weight: 90 kDa, 78 kDa
• GenBank Accession Number: BC000184
• Gene Symbol: PRPF3
• Gene ID (NCBI): 9129
• RRID: AB_2170994
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: O43395
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The pre-mRNA splicing occurs in spliceosomes, which consist of four ribonucleoprotein (snRNP) particles (U1, U2, U5 and U4/U6) and more than fifty proteins. This U4/U6-associated splicing factor, Hprp3p, is a 77 kDa protein, which is homologous to the Saccharomyces cerevisiae splicing factor Prp3p and may play a role in spliceosome assembly. Western blot analysis of HeLa cell nuclear extracts detected PRP3 protein at an apparent molecular mass of about 90 kD(PMID: 9328476 ). The discrepancy between the molecular mass reported here and that predicted from the Hprp3p coding sequence can be attributed, at least partially, to the highly positive charge; its value of predicted isoelectric point (IP) is ∼9.99. This antibody is a rabbit polyclonal antibody raised against an internal region of human PRPF3. A predicted molecular mass of 77 kDa and the gene is named PRPF3 because it is homologous to the yeast PRP3 gene. We noticed that although the predicted molecular mass of PRPF3 is77 kDa, the protein produced in E.coli migrated like a 90 kDa protein in SDS-PAGE. (PMID: 9328476)

Publications

Species	Application	Title
human	WB	Hum Mol Genet; PRPF mutations are associated with generalized defects in spliceosome formation and pre-mRNA splicing in patients with retinitis pigmentosa.; Authors - Tanackovic Goranka G
human	WB	Mol Cell Biol; A quantitative high-throughput in vitro splicing assay identifies inhibitors of spliceosome catalysis.; Authors - Berg Michael G MG
human	WB	J Biol Chem; PRPF19 regulates p53-dependent cellular senescence by modulating alternative splicing of MDM4 mRNA.; Authors - Kimiyoshi Yano
human	IF	Cell Mol Biol Lett; TMEM43 promotes pancreatic cancer progression by stabilizing PRPF3 and regulating RAP2B/ERK axis.; Authors - Junqiang Li
mouse	WB	Food Funct; Flaxseed oil attenuates PFOS-induced testicular damage by regulating RNA alternative splicing; Authors - Jingyi Ren