PRPF19 Polyclonal antibody

PRPF19 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

3

Cat no : 15414-1-AP

Synonyms

RING-type E3 ubiquitin transferase PRP19, PSO4, PRP19, Pre-mRNA-processing factor 19, hPso4

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Validation Data Gallery

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  WB analysis of mouse kidney using 15414-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 15414-1-AP (PRPF19 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat kidney using 15414-1-AP

  rat kidney tissue were subjected to SDS PAGE followed by western blot with 15414-1-AP (PRPF19 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 15414-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 15414-1-AP (PRPF19 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 15414-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 15414-1-AP (PRPF19 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human testis using 15414-1-AP

  human testis tissue were subjected to SDS PAGE followed by western blot with 15414-1-AP (PRPF19 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-3 using 15414-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 15414-1-AP (PRPF19 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 15414-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 15414-1-AP (PRPF19 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human stomach using 15414-1-AP

  Immunohistochemical analysis of paraffin-embedded human normal stomach slide using 15414-1-AP (PRPF19 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: mouse kidney tissue, HEK-293 cells, HeLa cells, human testis tissue, mouse liver tissue, PC-3 cells, rat kidney tissue
• Positive IHC detected in: human stomach tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 3 publications below

Product Information

15414-1-AP targets PRPF19 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, pig
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PRPF19 fusion protein Ag7700
• Full Name: PRP19/PSO4 pre-mRNA processing factor 19 homolog (S. cerevisiae)
• Calculated Molecular Weight: 55 kDa
• Observed Molecular Weight: 55 kDa
• GenBank Accession Number: BC008719
• Gene Symbol: PRPF19
• Gene ID (NCBI): 27339
• RRID: AB_10637855
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PRPF19 is involved in several processes, including DNA damage checkpoint signaling; cellular protein metabolic process; and mRNA splicing, via spliceosome.

Publications

Species	Application	Title
mouse	WB	Expert Opin Ther Targets; Proteome of thymus and spleen reveals that 10-hydroxydec-2-enoic acid could enhance immunity in mice.; Authors - Pei Fan
human	WB	J Cell Biochem; Human RNF113A participates of pre-mRNA splicing in vitro.; Authors - Guilherme H Gatti da Silva
human,pig	WB	J Virol; PRPF19 Limits Porcine Epidemic Diarrhea Virus Replication through Targeting and Degrading Viral Capsid Protein; Authors - Xueying Zhai