PROS1 Polyclonal antibody

PROS1 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IHC, IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

Publications

14

Reviews (1)

Cat no : 16910-1-AP

Synonyms

Vitamin K-dependent protein S, PROS

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Validation Data Gallery

• 

  WB analysis of human placenta using 16910-1-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 16910-1-AP (PROS1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 16910-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16910-1-AP (PROS1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse placenta using 16910-1-AP

  mouse placenta tissue were subjected to SDS PAGE followed by western blot with 16910-1-AP (PROS1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human placenta using 16910-1-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 16910-1-AP (PROS1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human liver using 16910-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 16910-1-AP (PROS1 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human liver using 16910-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 16910-1-AP (PROS1 antibody) at dilution of 1:100 (under 40x lens).

• 

  IF Staining of HepG2 using 16910-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using 16910-1-AP (PROS1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

• 

  FC experiment of HepG2 using 16910-1-AP

  1X10^6 HepG2 cells were intracellularly stained with 0.4 ug Anti-Human PROS1 (16910-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

• Positive WB detected in: human placenta tissue, DU 145 cells, mouse placenta tissue, HepG2 cells, HuH-7 cells
• Positive IHC detected in: human liver tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells
• Positive FC (Intra) detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 9 publications below
• IHC: See 3 publications below
• IF: See 5 publications below
• ELISA: See 1 publications below
• FC: See 1 publications below

Product Information

16910-1-AP targets PROS1 in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse, plasmodium falciparum
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PROS1 fusion protein Ag10539
• Full Name: protein S (alpha)
• Calculated Molecular Weight: 75 kDa
• Observed Molecular Weight: 70 kDa
• GenBank Accession Number: BC015801
• Gene Symbol: PROS1
• Gene ID (NCBI): 5627
• RRID: AB_2300207
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PROS1, also named as Vitamin K-dependent protein S, is a 676 amino acid protein, which contains 4 EGF-like domains, contains one Gla (gamma-carboxy-glutamate) domain and contains 2 laminin G-like domains. PROS1 as a secreted protein platelets alpha granules is expressed in plasma. PROS1 is a cofactor to activated protein C in the degradation of coagulation factors Va and VIIIa and helps to prevent coagulation and stimulating fibrinolysis.

Publications

Species	Application	Title
mouse	FC	Science; The TAM family receptor tyrosine kinase TYRO3 is a negative regulator of type 2 immunity.; Authors - Pamela Y Chan
		Brain; Extracellular vesicle biomarkers for complement dysfunction in schizophrenia; Authors - Ting Xue
human	WB,IHC	Aging (Albany NY); CeRNA regulatory network-based analysis to study the roles of noncoding RNAs in the pathogenesis of intrahepatic cholangiocellular carcinoma.; Authors - Weiyu Xu
human	WB	Mol Cell Proteomics; Multisample Mass Spectrometry-Based Approach for Discovering Injury Markers in Chronic Kidney Disease.; Authors - Ji Eun Kim
mouse	WB	Front Cell Neurosci; Proteomic and Metabolomic Analyses of Vanishing White Matter Mouse Astrocytes Reveal Deregulation of ER Functions.; Authors - Lisanne E Wisse
mouse	IF	Am J Physiol Renal Physiol; Identification of natriuretic peptide receptor A-related gene expression signatures in podocytes in vivo reveals baseline control of protective pathways; Authors - Mia Jensen

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Hongxue (Verified Customer) (01-25-2021)

Nice band for WB

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Liver

PROS1 Antibody Western Blot validation (1:1000 dilution) in Liver (Cat no:16910-1-AP)

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