Prokineticin 1 Polyclonal antibody

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  IHC staining of human colon cancer using 15152-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 15152-1-AP (Prokineticin 1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse kidney using 15152-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 15152-1-AP (Prokineticin 1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse kidney using 15152-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 15152-1-AP (Prokineticin 1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse liver using 15152-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 15152-1-AP (Prokineticin 1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse liver using 15152-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 15152-1-AP (Prokineticin 1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 15152-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 15152-1-AP (Prokineticin 1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 15152-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 15152-1-AP (Prokineticin 1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 15152-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 15152-1-AP (Prokineticin 1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse kidney using 15152-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 15152-1-AP (Prokineticin 1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of NIH/3T3 using 15152-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed NIH/3T3 cells using Prokineticin 1 antibody (15152-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

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  FC experiment of NIH/3T3 using 15152-1-AP

  1X10^6 NIH/3T3 cells were intracellularly stained with 0.4 ug Anti-Human Prokineticin 1 (15152-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP, Clone: ) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Prokineticin 1 Polyclonal Antibody for IHC, IF/ICC, FC (Intra), ELISA

Cat No. 15152-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IHC, IF/ICC, FC (Intra), ELISA

Synonyms

PROK1, Mambakine, Endocrine-gland-derived vascular endothelial growth factor, EG-VEGF, EGVEGF

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Tested Applications

• Positive IHC detected in: human colon cancer tissue, mouse kidney tissue, mouse liver tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: NIH/3T3 cells
• Positive FC (Intra) detected in: NIH/3T3 cells

Recommended dilution

• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

15152-1-AP targets Prokineticin 1 in IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Prokineticin 1 fusion protein Ag5098
• Full Name: prokineticin 1
• Calculated Molecular Weight: 12 kDa
• GenBank Accession Number: BC025399
• Gene Symbol: Prokineticin 1
• Gene ID (NCBI): 84432
• RRID: AB_3669199
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P58294
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Prokineticin 1 (PROK1) is also named as EG-VEGF and Mambakine, belongs to the to the AVIT (prokineticin) family. Prokineticin signaling comprises two secreted proteins (Prok-1 and Prok-2) and two cognate G-protein coupled receptors (PK-R1 and PK-R2) that are widely expressed in different tissues and of great versatility. Prokineticins were shown to promote angiogenesis in steroidgenic glands, heart and reproductive organs (PMID:18440852). PROK1 has been described as a secretory protein with pleiotropic functions and as a novel tissue-specific angiogenic factor (PMID:32355954). EG-VEGF/PK-1, described as selective angiogenic mitogen, is widely expressed in different tissues including steroidogenic endocrine glands (PMID:16320832). A lot of data suggests EG-VEGF to be restricted to endocrine glands and to some endocrine-dependent organs (PMID:28386275).