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PRMT7 Monoclonal antibody, PBS Only

PRMT7 Monoclonal Antibody for WB, IF, Indirect ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human, rat

Applications

WB, IF, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

3C5H6

Cat no : 67669-1-PBS

Synonyms

FLJ10640, KIAA1933, PRMT7

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using PRMT7 Monoclonal antibody (67669-1-Ig)

    WB analysis using 67669-1-Ig (same clone as 67669-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67669-1-Ig (PRMT7 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67669-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HSC-T6 cells using PRMT7 Monoclonal antibody (67669-1-Ig)

    WB analysis of HSC-T6 using 67669-1-Ig (same clone as 67669-1-PBS)

    HSC-T6 cells were subjected to SDS PAGE followed by western blot with 67669-1-Ig (PRMT7 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67669-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HepG2 cells using PRMT7 Monoclonal antibody (67669-1-Ig)

    WB analysis of HepG2 using 67669-1-Ig (same clone as 67669-1-PBS)

    HepG2 cells were subjected to SDS PAGE followed by western blot with 67669-1-Ig (PRMT7 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67669-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of Jurkat cells using PRMT7 Monoclonal antibody (67669-1-Ig)

    IF Staining of Jurkat using 67669-1-Ig (same clone as 67669-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using PRMT7 antibody (67669-1-Ig, Clone: 3C5H6 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67669-1-PBS in a different storage buffer formulation.

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67669-1-PBS targets PRMT7 in WB, IF, Indirect ELISA applications and shows reactivity with Human, rat samples.

Tested Reactivity Human, rat
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen PRMT7 fusion protein Ag30434
Full Name protein arginine methyltransferase 7
Calculated Molecular Weight 78 kDa
Observed Molecular Weight78 kDa
GenBank Accession NumberBC000146
Gene Symbol PRMT7
Gene ID (NCBI) 54496
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Protein arginine methyltransferases (PRMTs) are responsible for arginine methylation of histone or nonhistone proteins to regulate diverse biological processes, including cell proliferation, differentiation and senescence. PRMT7 is expressed in both embryonic stem and germ cells and is implicated in maintenance of muscle stem cell function. overexpression of PRMT7 has been associated with cancer metastasis. PRMT7 automethylation at residue R531 was reported to enhance breast cancer metastasis.