PRMT5 Monoclonal antibody

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  WB analysis using 68534-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68534-1-Ig (PRMT5 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

PRMT5 Monoclonal Antibody for WB, ELISA

Cat No. 68534-1-Ig

Clone No.4A1B5

Host / Isotype

Mouse / IgG1

Reactivity

Human, rat

Applications

WB, ELISA

Synonyms

72 kDa ICln binding protein, HRMT1L5, IBP72, Jak binding protein 1, JBP1, PRMT5, SKB1, SKB1 homolog, SKB1Hs

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Tested Applications

• Positive WB detected in: MCF-7 cells, HeLa cells, HEK-293 cells, HepG2 cells, Jurkat cells, HSC-T6 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

68534-1-Ig targets PRMT5 in WB, ELISA applications and shows reactivity with Human, rat samples.

• Tested Reactivity: Human, rat
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: PRMT5 fusion protein Ag9678
• Full Name: protein arginine methyltransferase 5
• Calculated Molecular Weight: 637 aa, 73 kDa
• Observed Molecular Weight: 68 kDa
• GenBank Accession Number: BC025979
• Gene Symbol: PRMT5
• Gene ID (NCBI): 10419
• RRID: AB_3085242
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• UNIPROT ID: O14744
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PRMT5 is a Type II enzyme that catalyzes arginine monomethylation and symmetric dimethylation (Rme1 and Rme2s). The many targets of PRMT5 include ribosomal proteins, the histone chaperone nucleoplasmin, p53, and histones. PRMT5 is frequently observed in a complex with the cofactor, methylosome protein 50 (MEP50), which is required for PRMT5 activity. PRMT5 is upregulated in several human malignancies, including lymphomas, lung cancer, breast cancer and colorectal cancer.