PRMT3 Recombinant antibody, PBS Only (Detector)

PRMT3 Uni-rAb^TM Recombinant Antibody for IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

241092F2

Cat no : 83969-3-PBS

Synonyms

Protein arginine N-methyltransferase 3, PRMT 3, HRMT1L3, Heterogeneous nuclear ribonucleoprotein methyltransferase-like protein 3, EC:2.1.1.319

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Validation Data Gallery

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  IF Staining of HeLa using 83969-3-RR (same clone as 83969-3-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using PRMT3 antibody (83969-3-RR, Clone: 241092F2 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 83969-3-PBS in a different storage buffer formulation.

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  FC experiment of A549 using 83969-3-RR (same clone as 83969-3-PBS)

  1x10^6 A549 cells were intracellularly stained with 0.25 ug PRMT3 Recombinant antibody (83969-3-RR, Clone:241092F2) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83969-3-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP00892-1

  Cytometric bead array standard curve of MP00892-1, PRMT3 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83969-5-PBS. Detection antibody: 83969-3-PBS. Standard: Ag13466. Range: 0.781-100 ng/mL.

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  Cytometric bead array standard curve of MP00892-3

  Cytometric bead array standard curve of MP00892-3, PRMT3 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83969-2-PBS. Detection antibody: 83969-3-PBS. Standard: Ag13466. Range: 0.781-100 ng/mL.

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Product Information

83969-3-PBS targets PRMT3 as part of a matched antibody pair:

MP00892-1: 83969-5-PBS capture and 83969-3-PBS detection (validated in Cytometric bead array)

MP00892-3: 83969-2-PBS capture and 83969-3-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: PRMT3 fusion protein Ag13466
• Full Name: protein arginine methyltransferase 3
• Calculated Molecular Weight: 531 aa, 60 kDa
• GenBank Accession Number: BC037544
• Gene Symbol: PRMT3
• Gene ID (NCBI): 10196
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

PRMT3(protein arginine methyltransferase 3) regulates protein functions by introducing asymmetric dimethylation marks at the arginine residues in proteins.