PRM2 Polyclonal antibody, PBS Only

• 

  WB analysis of human testis using 14500-1-AP (same clone as 14500-1-PBS)

  human testis tissue were subjected to SDS PAGE followed by western blot with 14500-1-AP (PRM2 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14500-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human testis using 14500-1-AP (same clone as 14500-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 14500-1-AP (PRM2 antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14500-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human testis using 14500-1-AP (same clone as 14500-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 14500-1-AP (PRM2 antibody) at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14500-1-PBS in a different storage buffer formulation.

PRM2 Polyclonal Antibody for WB, IHC, Indirect ELISA

Cat No. 14500-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, Indirect ELISA

Synonyms

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Product Information

14500-1-PBS targets PRM2 in WB, IHC, Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PRM2 fusion protein Ag5952
• Full Name: protamine 2
• Calculated Molecular Weight: 13 kDa
• Observed Molecular Weight: 10-25 kDa
• GenBank Accession Number: BC066338
• Gene Symbol: PRM2
• Gene ID (NCBI): 5620
• RRID: AB_2721024
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P04554
• Storage Buffer: PBS only , pH 7.3
• Storage Conditions: Store at -80°C.

Background Information

PRM2 (Protamine-2) is the main DNA-binding protein in the sperm nucleus, consisting of a C-terminal mature PRM2 (mPRM2) domain and an N-terminal lytic PRM2 (cPRM2) domain, which is lyzed sequentially after binding to DNA. PRM2 plays an important role in spermatogenesis and development. PRM2 is specifically expressed in sperm and localized in the sperm nucleus.