PRKRA Monoclonal antibody, PBS Only

PRKRA Monoclonal Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1D4C3

Cat no : 68518-1-PBS

Synonyms

PACT, Interferon-inducible double-stranded RNA-dependent protein kinase activator A, HSD-14, HSD14, HSD 14

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Validation Data Gallery

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  WB analysis using 68518-1-Ig (same clone as 68518-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68518-1-Ig (PRKRA antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68518-1-PBS in a different storage buffer formulation.

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  IHC staining of human ovary cancer using 68518-1-Ig (same clone as 68518-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 68518-1-Ig (PRKRA antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68518-1-PBS in a different storage buffer formulation.

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  IHC staining of human ovary cancer using 68518-1-Ig (same clone as 68518-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 68518-1-Ig (PRKRA antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68518-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 68518-1-Ig (same clone as 68518-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 68518-1-Ig (PRKRA antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68518-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brain using 68518-1-Ig (same clone as 68518-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 68518-1-Ig (PRKRA antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68518-1-PBS in a different storage buffer formulation.

• 

  IHC staining of rat brain using 68518-1-Ig (same clone as 68518-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 68518-1-Ig (PRKRA antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68518-1-PBS in a different storage buffer formulation.

• 

  IHC staining of rat brain using 68518-1-Ig (same clone as 68518-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 68518-1-Ig (PRKRA antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68518-1-PBS in a different storage buffer formulation.

• 

  IHC staining of rat cerebellum using 68518-1-Ig (same clone as 68518-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue slide using 68518-1-Ig (PRKRA antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68518-1-PBS in a different storage buffer formulation.

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Product Information

68518-1-PBS targets PRKRA in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen: PRKRA fusion protein Ag29818
• Full Name: protein kinase, interferon-inducible double stranded RNA dependent activator
• Calculated Molecular Weight: 34 kDa
• Observed Molecular Weight: 34 kDa
• GenBank Accession Number: BC009470
• Gene Symbol: PRKRA
• Gene ID (NCBI): 8575
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

PRKRA (PKR-associated protein X) is also named as PACT, RAX, HSD-14 and HSD14. PRKRA was significantly overexpressed in PC tissues and promoted the proliferation, migration, and chemoresistance of PC cells (PMID: 37484321). PRKRA activates EIF2AK2/PKR in the absence of double-stranded RNA (dsRNA), leading to phosphorylation of EIF2S1/EFI2-alpha and inhibition of translation and induction of apoptosis. PRKRA has been shown to interact with DICER1. DICER1 and PRKRA expression in normal colon was limited to mucosal epithelium and showed similar cytoplasmic expression (PMID: 37484321).