PACT Polyclonal antibody

• Featured Product
• KD/KO Validated

PACT Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, CoIP, ELISA

Conjugate

Unconjugated

Publications

12

Cat no : 10771-1-AP

Synonyms

DYT16, HSD 14, HSD14, PACT, PKR associated protein X, PKR associating protein X, PRKRA, RAX

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Validation Data Gallery

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  WB analysis using 10771-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10771-1-AP (PACT antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of MCF-7 using 10771-1-AP

  WB result of PACT antibody (10771-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PACT transfected MCF-7 cells.

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  WB analysis of HeLa using 10771-1-AP

  WB result of PACT antibody (10771-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PACT transfected HeLa cells.

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  IHC staining of mouse testis using 10771-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 10771-1-AP (PACT antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse testis using 10771-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 10771-1-AP (PACT antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: HeLa cells, MCF-7 cells, C2C12 cells, PC-12 cells
• Positive IHC detected in: mouse testis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 9 publications below
• IHC: See 1 publications below
• IF: See 3 publications below
• CoIP: See 1 publications below

Product Information

10771-1-AP targets PACT in WB, IHC, IF, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PACT fusion protein Ag1214
• Full Name: protein kinase, interferon-inducible double stranded RNA dependent activator
• Calculated Molecular Weight: 34 kDa
• Observed Molecular Weight: 31-34 kDa
• GenBank Accession Number: BC009470
• Gene Symbol: PRKRA
• Gene ID (NCBI): 8575
• RRID: AB_2170496
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PRKRA (PKR-associated protein X) is also named as PACT, RAX, HSD-14 and HSD14. PRKRA was significantly overexpressed in PC tissues and promoted the proliferation, migration, and chemoresistance of PC cells (PMID: 37484321). PRKRA activates EIF2AK2/PKR in the absence of double-stranded RNA (dsRNA), leading to phosphorylation of EIF2S1/EFI2-alpha and inhibition of translation and induction of apoptosis. PRKRA has been shown to interact with DICER1. DICER1 and PRKRA expression in normal colon was limited to mucosal epithelium and showed similar cytoplasmic expression (PMID: 37484321).

Publications

Species	Application	Title
human	WB	Cell Rep; LINC00921 reduces lung cancer radiosensitivity by destabilizing NUDT21 and driving aberrant MED23 alternative polyadenylation; Authors - Nasha Zhang
rat	IF	EMBO Rep; The dynamic recruitment of TRBP to neuronal membranes mediates dendritogenesis during development.; Authors - Anna Antoniou
human	WB	Nucleic Acids Res; Hepato-specific microRNA-122 facilitates accumulation of newly synthesized miRNA through regulating PRKRA.; Authors - Li Shuai S
human	WB	PLoS Pathog; KSHV inhibits stress granule formation by viral ORF57 blocking PKR activation.; Authors - Nishi R Sharma
human	WB	J Proteome Res; Proteomic analysis of early HIV-1 nucleoprotein complexes.; Authors - Schweitzer Cameron J CJ
human,mouse	WB,IF	Dis Model Mech; A frameshift mutation in the murine Prkra gene exhibits cerebellar abnormality and reduced eIF2α phosphorylation; Authors - Samuel B Burnett