PRKG2 Polyclonal antibody

• Featured Product
• KD/KO Validated

PRKG2 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

11

Cat no : 55138-1-AP

Synonyms

cGK 2, cGK2, cGKII, PRKG2, PRKGR2

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Validation Data Gallery

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  WB analysis using 55138-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 55138-1-AP (PRKG2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 55138-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 55138-1-AP (PRKG2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 55138-1-AP

  IP result of anti-PRKG2 (IP:55138-1-AP, 4ug; Detection:55138-1-AP 1:500) with mouse brain tissue lysate 4000ug.

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  IHC staining of human colon using 55138-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon using 55138-1-AP (PRKG2 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human colon using 55138-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon using 55138-1-AP (PRKG2 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human lung using 55138-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 55138-1-AP (PRKG2 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human lung using 55138-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 55138-1-AP (PRKG2 antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of HUVEC using 55138-1-AP

  Immunofluorescent analysis of HUVEC cells using 55138-1-AP (PRKG2 antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG.

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  IF Staining of C6 using 55138-1-AP

  Immunofluorescent analysis of C6 cells using 55138-1-AP (PRKG2 antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG.

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Tested Applications

• Positive WB detected in: mouse brain tissue, rat brain tissue
• Positive IP detected in: mouse brain tissue
• Positive IHC detected in: human colon tissue, human lung tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HUVEC cells, C6 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 3 publications below
• WB: See 10 publications below
• IHC: See 1 publications below
• IF: See 1 publications below
• IP: See 1 publications below

Product Information

55138-1-AP targets PRKG2 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: protein kinase, cGMP-dependent, type II
• Calculated Molecular Weight: 87 kDa
• Observed Molecular Weight: 85-90 kDa
• GenBank Accession Number: NM_006259
• Gene Symbol: PRKG2
• Gene ID (NCBI): 5593
• RRID: AB_10804353
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PRKG2, also named as PRKGR2 and cGK2, belongs to the protein kinase superfamily, AGC Ser/Thr protein kinase family and cGMP subfamily. It catalyses the reaction: ATP + a protein = ADP + a phosphoprotein. The antibody is specific to PRKG2.

Publications

Species	Application	Title
mouse	WB	EMBO Rep; Phosphorylation of myelin regulatory factor by PRKG2 mediates demyelination in Huntington's disease.; Authors - Peng Yin ; KO Validated
human	WB	Front Oncol; High-Risk HPV16 E6 Activates the cGMP/PKG Pathway Through Glycosyltransferase ST6GAL1 in Cervical Cancer Cells.; Authors - Jun Wang
human	WB	Nutr Cancer; Erianin Exerts Antineoplastic Effects on Esophageal Squamous Cell Carcinoma Cells by Activating the cGMP-PKG Signaling Pathway; Authors - Xin Deng
human	WB	Cell Death Dis; The novel ZEB1-upregulated protein PRTG induced by Helicobacter pylori infection promotes gastric carcinogenesis through the cGMP/PKG signaling pathway.; Authors - Tian Xiang
human	WB	Physiol Rep; Linaclotide activates guanylate cyclase-C/cGMP/protein kinase-II-dependent trafficking of CFTR in the intestine.; Authors - Md Kaimul Ahsan ; KD Validated
mouse	WB	Phytomedicine; Sambucus williamsii Hance maintains bone homeostasis in hyperglycemia-induced osteopenia by reversing oxidative stress via cGMP/PKG signal transduction; Authors - Yi-Wei Shen ; KD Validated