PRKG1 Polyclonal antibody

• Featured Product
• KD/KO Validated

PRKG1 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, rat, mouse and More (1)

Applications

WB, IHC, IP, ELISA

Conjugate

Unconjugated

Publications

19

Cat no : 21646-1-AP

Synonyms

cGK 1, cGK1, CGKI, cGKI alpha, cGKI BETA, PGK, PKG, PRKG1, PRKG1B, PRKGR1A, PRKGR1B, Protein Kinase G1 alpha

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Validation Data Gallery

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  WB analysis using 21646-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 21646-1-AP (PRKG1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis using 21646-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 21646-1-AP (PRKG1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of rat brain using 21646-1-AP

  rat brain tissue were subjected to SDS PAGE followed by western blot with 21646-1-AP (PRKG1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 21646-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 21646-1-AP (PRKG1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 21646-1-AP

  IP result of anti-PRKG1 (IP:21646-1-AP, 4ug; Detection:21646-1-AP 1:600) with mouse brain tissue lysate 4000ug.

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  IHC staining of human heart using 21646-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 21646-1-AP (PRKG1 Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human heart using 21646-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 21646-1-AP (PRKG1 Antibody) at dilution of 1:200 (under 40x lens).

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Tested Applications

• Positive WB detected in: mouse brain tissue, rat brain tissue, mouse kidney tissue, mouse lung tissue
• Positive IP detected in: mouse brain tissue
• Positive IHC detected in: human heart tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:3000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 19 publications below
• IHC: See 2 publications below

Product Information

The immunogen of 21646-1-AP is PRKG1 Fusion Protein expressed in E. coli.

• Tested Reactivity: human, rat, mouse
• Cited Reactivity: human, mouse, rat, bovine
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PRKG1 fusion protein Ag16333
• Full Name: protein kinase, cGMP-dependent, type I
• Calculated Molecular Weight: 686 aa, 78 kDa
• Observed Molecular Weight: 65-78 kDa
• GenBank Accession Number: BC127090
• Gene Symbol: PRKG1
• Gene ID (NCBI): 5592
• RRID: AB_2878897
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PRKG1(cGMP-dependent protein kinase 1) is also named as cGK1, PRKG1B, PRKGR1A, PRKGR1B and belongs to the cGMP subfamily. It serves as an integral component of second messenger signaling in a number of biological contexts including cell differentiation, memory, and vasodilation(PMID:21893290). Its activity prevents pathological-level nitric oxide-induced apoptosis and promotes DNA synthesis/cell proliferation in vascular smooth muscle cells(PMID:20060325). PRKG1 has 2 isoforms produced by alternative splicing with the molecular weight of 76 kDa and 78 kDa. The autophosphorylation can increase the kinase activity and it can form a monomer with the molecular weight of 65 kDa that is produced by proteolytic cleavage. SDS-PAGE revealed that proteolysis generated two different monomers with molecular masses of 70 and 68 kDa of CGK1-beta(PMID:8702828).

Publications

Species	Application	Title
rat	WB	Environ Pollut; Fluoride-induced unrestored arrest during haploid period of spermatogenesis via the regulation of DDX25 in rats.; Authors - Yongli Han
mouse	WB	Clin Sci (Lond); Empagliflozin prevents cardiomyopathy via sGC-cGMP-PKG pathway in type 2 diabetes mice.; Authors - Mei Xue
bovine	WB	Front Cell Dev Biol; Myostatin Mutation Promotes Glycolysis by Increasing Phosphorylation of Phosphofructokinase via Activation of PDE5A-cGMP-PKG in Cattle Heart.; Authors - Mingjuan Gu ; KD Validated
rat	WB	Front Pharmacol; Emodin Interferes With Nitroglycerin-Induced Migraine in Rats Through CGMP-PKG Pathway.; Authors - Shuding Sun
human	WB	Cancer Cell Int; NEK7 promotes gastric cancer progression as a cell proliferation regulator.; Authors - Yi-Ke Li
rat	WB	Eur J Pharmacol; A novel phosphodiesterase 9A inhibitor LW33 protects against ischemic stroke through the cGMP/PKG/CREB pathway.; Authors - Jia-Yi You