DNA-PKcs Polyclonal antibody

DNA-PKcs Polyclonal Antibody for WB, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (2)

Applications

WB, IHC, IF, IP, CoIP, ELISA

Conjugate

Unconjugated

Publications

20

Reviews (1)

Cat no : 19983-1-AP

Synonyms

DNA PK catalytic subunit, DNAPK, DNA-PKcs, DNPK1, HYRC, HYRC1, p350, p460, PRKDC, XRCC7

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Validation Data Gallery

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  WB analysis using 19983-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 19983-1-AP (DNA-PKcs antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 19983-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 19983-1-AP (DNA-PKcs antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 19983-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 19983-1-AP (DNA-PKcs antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 19983-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 19983-1-AP (DNA-PKcs antibody at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 19983-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 19983-1-AP (DNA-PKcs antibody at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HeLa using 19983-1-AP

  IP result of anti-DNA-PKcs (IP:19983-1-AP, 4ug; Detection:19983-1-AP 1:500) with HeLa cells lysate 1320 ug.

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Tested Applications

• Positive WB detected in: HeLa cells, MCF-7 cells
• Positive IP detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 18 publications below
• IHC: See 2 publications below
• IF: See 1 publications below
• CoIP: See 1 publications below

Product Information

19983-1-AP targets DNA-PKcs in WB, IHC, IF, IP, CoIP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: protein kinase, DNA-activated, catalytic polypeptide
• Calculated Molecular Weight: 469 kDa
• Observed Molecular Weight: 350-460 kDa
• GenBank Accession Number: NM_006904
• Gene Symbol: PRKDC
• Gene ID (NCBI): 5591
• RRID: AB_10642811
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PRKDC, also named as HYRC, HYRC1, DNPK1 and p460, belongs to the PI3/PI4-kinase family. PRKDC is a serine/threonine-protein kinase that acts as a molecular sensor for DNA damage. Involved in DNA nonhomologous end joining (NHEJ), PRKDC is required for double-strand break (DSB) repair and V(D)J recombination. PRKDC must be bound to DNA to express its catalytic properties. It promotes processing of hairpin DNA structures in V(D)J recombination by activation of the hairpin endonuclease artemis (DCLRE1C). It is required to protect and align broken ends of DNA. PRKDC may also act as a scaffold protein to aid the localization of DNA repair proteins to the site of damage. It is found at the ends of chromosomes, suggesting a further role in the maintenance of telomeric stability and the prevention of chromosomal end fusion. It also involved in modulation of transcription. It recognizes the substrate consensus sequence [ST]-Q. PRKDC phosphorylates 'Ser-139' of histone variant H2AX/H2AFX, thereby regulating DNA damage response mechanism. It phosphorylates DCLRE1C, c-Abl/ABL1, histone H1, HSPCA, c-jun/JUN, p53/TP53, PARP1, POU2F1, DHX9, SRF, XRCC1, XRCC1, XRCC4, XRCC5, XRCC6, WRN, c-myc/MYC and RFA2. The antibody recognizes the C-term of PRKDC.

Publications

Species	Application	Title
human	WB	Science; IFI16 DNA sensor is required for death of lymphoid CD4 T cells abortively infected with HIV.; Authors - Kathryn M Monroe
human	WB	Nat Commun; Endonuclease G promotes autophagy by suppressing mTOR signaling and activating the DNA damage response.; Authors - Wenjun Wang
human	IF, WB	J Cell Biol; DNA damage promotes microtubule dynamics through a DNA-PK-AKT axis for enhanced repair.; Authors - Shuyun Ma
human	WB	Int J Biol Macromol; Antitumor efficacy of a recombinant EGFR-targeted fusion protein conjugate that induces telomere shortening and telomerase downregulation; Authors - Hong-Yu Tao
human	WB,CoIP	Cancer Lett; ITGA2 overexpression inhibits DNA repair and confers sensitivity to radiotherapies in pancreatic cancer; Authors - Chen Zhou
human	WB	Cell Biol Toxicol; Long noncoding RNA lnc-RI regulates DNA damage repair and radiation sensitivity of CRC cells through NHEJ pathway.; Authors - Ruixue Liu

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

CHAO (Verified Customer) (03-31-2022)

Strong correct bands

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Mouse Heart