DNA-PKcs Polyclonal antibody

DNA-PKcs Polyclonal Antibody for WB, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (2)

Applications

WB, IHC, IF, IP, CoIP, ELISA

Conjugate

Unconjugated

Cat no : 19983-1-AP

Synonyms

DNA PK catalytic subunit, DNAPK, DNA-PKcs, DNPK1, HYRC, HYRC1, p350, p460, PRKDC, XRCC7



Tested Applications

Positive WB detected inHeLa cells, MCF-7 cells
Positive IP detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

19983-1-AP targets DNA-PKcs in WB, IHC, IF, IP, CoIP, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name protein kinase, DNA-activated, catalytic polypeptide
Calculated Molecular Weight 469 kDa
Observed Molecular Weight 350-460 kDa
GenBank Accession NumberNM_006904
Gene Symbol PRKDC
Gene ID (NCBI) 5591
RRIDAB_10642811
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PRKDC, also named as HYRC, HYRC1, DNPK1 and p460, belongs to the PI3/PI4-kinase family. PRKDC is a serine/threonine-protein kinase that acts as a molecular sensor for DNA damage. Involved in DNA nonhomologous end joining (NHEJ), PRKDC is required for double-strand break (DSB) repair and V(D)J recombination. PRKDC must be bound to DNA to express its catalytic properties. It promotes processing of hairpin DNA structures in V(D)J recombination by activation of the hairpin endonuclease artemis (DCLRE1C). It is required to protect and align broken ends of DNA. PRKDC may also act as a scaffold protein to aid the localization of DNA repair proteins to the site of damage. It is found at the ends of chromosomes, suggesting a further role in the maintenance of telomeric stability and the prevention of chromosomal end fusion. It also involved in modulation of transcription. It recognizes the substrate consensus sequence [ST]-Q. PRKDC phosphorylates 'Ser-139' of histone variant H2AX/H2AFX, thereby regulating DNA damage response mechanism. It phosphorylates DCLRE1C, c-Abl/ABL1, histone H1, HSPCA, c-jun/JUN, p53/TP53, PARP1, POU2F1, DHX9, SRF, XRCC1, XRCC1, XRCC4, XRCC5, XRCC6, WRN, c-myc/MYC and RFA2. The antibody recognizes the C-term of PRKDC.

Protocols

Product Specific Protocols
WB protocol for DNA-PKcs antibody 19983-1-APDownload protocol
IP protocol for DNA-PKcs antibody 19983-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Science

IFI16 DNA sensor is required for death of lymphoid CD4 T cells abortively infected with HIV.

Authors - Kathryn M Monroe
humanWB

Nat Commun

Endonuclease G promotes autophagy by suppressing mTOR signaling and activating the DNA damage response.

Authors - Wenjun Wang
humanIF, WB

J Cell Biol

DNA damage promotes microtubule dynamics through a DNA-PK-AKT axis for enhanced repair.

Authors - Shuyun Ma
humanWB

Int J Biol Macromol

Antitumor efficacy of a recombinant EGFR-targeted fusion protein conjugate that induces telomere shortening and telomerase downregulation

Authors - Hong-Yu Tao
humanWB,CoIP

Cancer Lett

ITGA2 overexpression inhibits DNA repair and confers sensitivity to radiotherapies in pancreatic cancer

Authors - Chen Zhou
humanWB

Cell Biol Toxicol

Long noncoding RNA lnc-RI regulates DNA damage repair and radiation sensitivity of CRC cells through NHEJ pathway.

Authors - Ruixue Liu

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.


FH

CHAO (Verified Customer) (03-31-2022)

Strong correct bands

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: Mouse Heart