PRKD1 Recombinant antibody

PRKD1 Uni-rAb^TM Recombinant Antibody for IF/ICC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

230355D12

Cat no : 83174-5-RR

Synonyms

Serine/threonine protein kinase D1, PRKCM, PKD1, PKD, 230355D12

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Validation Data Gallery

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  IF Staining of HeLa using 83174-5-RR

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using PRKD1 antibody (83174-5-RR, Clone: 230355D12 ) at dilution of 1:100 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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  FC experiment of HeLa using 83174-5-RR

  1x10^6 HeLa cells were intracellularly stained with 0.25 ug Prkd1 Recombinant Antibody (83174-5-RR, Clone:230355D12) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

• Positive IF/ICC detected in: HeLa cells
• Positive FC (Intra) detected in: HeLa cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

83174-5-RR targets PRKD1 in IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Peptide
• Full Name: protein kinase D1
• Calculated Molecular Weight: 102 kDa
• GenBank Accession Number: NM_002742
• Gene Symbol: PRKD1
• Gene ID (NCBI): 5587
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.