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  • KD/KO Validated

PKC Iota Polyclonal antibody

PKC Iota Polyclonal Antibody for FC (Intra), IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG


human and More (2)


WB, IP, IHC, IF, FC (Intra), CoIP, ELISA



Cat no : 13883-1-AP


aPKC lambda/iota, DXS1179E, PKC iota, PKCI, PRKC lambda/iota, PRKCI, Protein kinase C iota type, protein kinase C, iota

Tested Applications

Positive WB detected inHeLa cells, MCF-7 cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman breast cancer tissue, human lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells
Positive FC detected inHeLa cells

Recommended dilution

Western Blot (WB)WB : 1:1000-1:6000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:200-1:800
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

13883-1-AP targets PKC Iota in WB, IP, IHC, IF, FC (Intra), CoIP, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman, mouse, chicken
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PKC Iota fusion protein Ag4990
Full Name protein kinase C, iota
Calculated Molecular Weight 68 kDa
Observed Molecular Weight 67 kDa
GenBank Accession NumberBC022016
Gene Symbol PRKCI
Gene ID (NCBI) 5584
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.


Product Specific Protocols
WB protocol for PKC Iota antibody 13883-1-APDownload protocol
IHC protocol for PKC Iota antibody 13883-1-APDownload protocol
IF protocol for PKC Iota antibody 13883-1-APDownload protocol
IP protocol for PKC Iota antibody 13883-1-APDownload protocol
FC protocol for PKC Iota antibody 13883-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols



Mol Cancer

circPARD3 drives malignant progression and chemoresistance of laryngeal squamous cell carcinoma by inhibiting autophagy through the PRKCI-Akt-mTOR pathway.

Authors - Wei Gao
  • KD Validated


Coupling of apical-basal polarity and PCP to interpret the Wnt signaling gradient and orient feather branch.

Authors - Jianqiong Lin
  • KD Validated

Mol Med

Paired protein kinases PRKCI-RIPK2 promote pancreatic cancer growth and metastasis via enhancing NF-κB/JNK/ERK phosphorylation

Authors - Juying Jiao

Int J Biol Sci

Poly (ADP-ribose) polymerase 1 (PARP1) inhibition promotes pulmonary metastasis of osteosarcoma by boosting ezrin phosphorylation.

Authors - Fangfei Li

Onco Targets Ther

Protein kinase C-iota-mediated glycolysis promotes non-small-cell lung cancer progression.

Authors - Liu Liu
  • KD Validated

Antioxidants (Basel)

PKCiota Inhibits the Ferroptosis of Esophageal Cancer Cells via Suppressing USP14-Mediated Autophagic Degradation of GPX4

Authors - Hao Tao
  • KD Validated