PKC Iota Polyclonal antibody

• Featured Product
• KD/KO Validated

PKC Iota Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (2)

Applications

WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ELISA

Conjugate

Unconjugated

Publications

7

Cat no : 13883-1-AP

Synonyms

PRKCI, nPKC-iota, EC:2.7.11.13, DXS1179E, Atypical protein kinase C-lambda/iota

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Validation Data Gallery

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  WB analysis using 13883-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13883-1-AP (PKC Iota antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of MCF-7 using 13883-1-AP

  WB result of PKC Iota antibody (13883-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PKC Iota transfected MCF-7 cells.

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  IP experiment of HeLa using 13883-1-AP

  IP result of anti-PKC Iota (IP:13883-1-AP, 4ug; Detection:13883-1-AP 1:2000) with HeLa cells lysate 1720 ug.

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  IHC staining of human breast cancer using 13883-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 13883-1-AP (PKC iota antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 13883-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 13883-1-AP (PKC iota antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 13883-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 13883-1-AP (PKC iota antibody at dilution of 1:200 (under 10x lens).

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  IHC staining of human lung cancer using 13883-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 13883-1-AP (PKC iota antibody at dilution of 1:200 (under 40x lens).

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  IF Staining of HeLa using 13883-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using PKC Iota antibody (13883-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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  FC experiment of HeLa using 13883-1-AP

  1X10^6 HeLa cells were intracellularly stained with 0.2 ug Anti-Human PKC Iota (13883-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.2 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

• Positive WB detected in: HeLa cells, MCF-7 cells
• Positive IP detected in: HeLa cells
• Positive IHC detected in: human breast cancer tissue, human lung cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells
• Positive FC (Intra) detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 4 publications below
• WB: See 6 publications below
• IHC: See 4 publications below
• IF: See 3 publications below
• IP: See 1 publications below
• CoIP: See 1 publications below

Product Information

13883-1-AP targets PKC Iota in WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse, chicken
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PKC Iota fusion protein Ag4990
• Full Name: protein kinase C, iota
• Calculated Molecular Weight: 68 kDa
• Observed Molecular Weight: 67 kDa
• GenBank Accession Number: BC022016
• Gene Symbol: PKC Iota
• Gene ID (NCBI): 5584
• RRID: AB_2171777
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB,IF	Mol Cancer; circPARD3 drives malignant progression and chemoresistance of laryngeal squamous cell carcinoma by inhibiting autophagy through the PRKCI-Akt-mTOR pathway.; Authors - Wei Gao ; KD Validated
chicken	IHC,IF	Development; Coupling of apical-basal polarity and PCP to interpret the Wnt signaling gradient and orient feather branch.; Authors - Jianqiong Lin ; KD Validated
human,mouse	WB,IHC,IF,CoIP	Mol Med; Paired protein kinases PRKCI-RIPK2 promote pancreatic cancer growth and metastasis via enhancing NF-κB/JNK/ERK phosphorylation; Authors - Juying Jiao
human	WB	Int J Biol Sci; Poly (ADP-ribose) polymerase 1 (PARP1) inhibition promotes pulmonary metastasis of osteosarcoma by boosting ezrin phosphorylation.; Authors - Fangfei Li
human	WB,IHC	Onco Targets Ther; Protein kinase C-iota-mediated glycolysis promotes non-small-cell lung cancer progression.; Authors - Liu Liu ; KD Validated
human	WB,IHC,IP	Antioxidants (Basel); PKCiota Inhibits the Ferroptosis of Esophageal Cancer Cells via Suppressing USP14-Mediated Autophagic Degradation of GPX4; Authors - Hao Tao ; KD Validated