PRKCDBP Polyclonal antibody

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  WB analysis of HeLa using 16250-1-AP

  WB result of PRKCDBP antibody (16250-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PRKCDBP transfected HeLa cells.

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  WB analysis of mouse lung using 16250-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 16250-1-AP (PRKCDBP antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  WB analysis of MCF-7 using 16250-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 16250-1-AP (PRKCDBP antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of A549 using 16250-1-AP

  IP result of anti-PRKCDBP (IP:16250-1-AP, 4ug; Detection:16250-1-AP 1:1000) with A549 cells lysate 640 ug.

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  IHC staining of human normal colon using 16250-1-AP

  Immunohistochemical analysis of paraffin-embedded human normal colon slide using 16250-1-AP (PRKCDBP antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of MCF-7 using 16250-1-AP

  Immunofluorescent analysis of MCF-7 cells, using PRKCDBP antibody 16250-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

• Featured Product
• KD/KO Validated

PRKCDBP Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 16250-1-AP

Publications(25)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF/ICC, IP, ELISA

Synonyms

Serum deprivation response factor-related gene product that binds to C-kinase, Protein kinase C delta-binding protein, hSRBC, Cavin-3, Cavin 3

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Tested Applications

• Positive WB detected in: mouse lung tissue, MCF-7 cells, HeLa cells
• Positive IP detected in: A549 cells
• Positive IHC detected in: human normal colon; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: MCF-7 cells

Recommended dilution

• Western Blot (WB): WB : 1:200-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 4 publications below
• WB: See 20 publications below
• IHC: See 4 publications below
• IF: See 7 publications below
• IP: See 1 publications below

Product Information

16250-1-AP targets PRKCDBP in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat, pig
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PRKCDBP fusion protein Ag9209
• Full Name: protein kinase C, delta binding protein
• Calculated Molecular Weight: 261 aa, 28 kDa
• Observed Molecular Weight: 35-40 kDa
• GenBank Accession Number: BC011585
• Gene Symbol: PRKCDBP
• Gene ID (NCBI): 112464
• RRID: AB_2171894
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q969G5
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Protein kinase C delta-binding protein (PRKCDBP) is a putative tumor suppressor in which alteration has been observed in several human cancers. It is a BRCA1-interacting protein, and may also be involved in DNA damage response and participate in BRCA1-mediated tumor suppression pathways. PKCdelta binds to the C-terminal half of PRKCDBP through the regulatory domain and phosphorylates it in vitro. In COS1 cells, the phosphorylation of over-expressed PRKCDBP is stimulated by 12-O-tetradecanoylphorbol-13-acetate and further enhanced by the over-expression of PKCdelta

Publications

Species	Application	Title
human	WB	ACS Nano; Engineering Extracellular Vesicles Restore the Impaired Cellular Uptake and Attenuate Intervertebral Disc Degeneration.; Authors - Zhiwei Liao
mouse	WB	PLoS Biol; Endocytic crosstalk: cavins, caveolins, and caveolae regulate clathrin-independent endocytosis.; Authors - Natasha Chaudhary
human	WB,IF	Nat Commun; Identification of intracellular cavin target proteins reveals cavin-PP1alpha interactions regulate apoptosis.; Authors - Kerrie-Ann McMahon ; KD Validated
human	WB,IF,IP	Dev Cell; Caveolae sense oxidative stress through membrane lipid peroxidation and cytosolic release of CAVIN1 to regulate NRF2; Authors - Yeping Wu
mouse	WB, IF	Elife; Cavin-3 dictates the balance between ERK and Akt signaling.; Authors - Victor J Hernandez
mouse	WB	Elife; Deciphering caveolar functions by syndapin III KO-mediated impairment of caveolar invagination.; Authors - Eric Seemann ; KO Validated