PKC Alpha Recombinant antibody, PBS Only

PKC Alpha Uni-rAb^TM Recombinant Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

240926E1

Cat no : 83840-6-PBS

Synonyms

PRKCA, PKC-A, PKCA, PKC A, EC:2.7.11.13

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Validation Data Gallery

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  WB analysis using 83840-6-RR (same clone as 83840-6-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 83840-6-RR (PKC Alpha antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83840-6-PBS in a different storage buffer formulation.

• 

  IHC staining of human stomach cancer using 83840-6-RR (same clone as 83840-6-PBS)

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 83840-6-RR (PKC Alpha antibody) at dilution of 1:800 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer(pH9). This data was developed using the same antibody clone with 83840-6-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 83840-6-RR (same clone as 83840-6-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using PRKCA antibody (83840-6-RR, Clone: 240926E1 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 83840-6-PBS in a different storage buffer formulation.

• 

  FC experiment of HepG2 using 83840-6-RR (same clone as 83840-6-PBS)

  1x10^6 HepG2 cells were intracellularly stained with 0.25 ug PRKCA Recombinant antibody (83840-6-RR, Clone:240926E1) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83840-6-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 83840-6-RR

  Biolayer interferometry (BLl) kinetic assays of 83840-6-RR against Human PRKCA were performed. The affinity constant is below 1 pM.

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Product Information

The immunogen of 83840-6-PBS is PKC Alpha Fusion Protein expressed in E. coli.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: PKC Alpha fusion protein Ag1025
• Full Name: protein kinase C, alpha
• Observed Molecular Weight: 77 kDa
• GenBank Accession Number: BC109274
• Gene Symbol: PKC Alpha
• Gene ID (NCBI): 5578
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and the second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC family members also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play a distinct role in cells. PRKCA is one of the PKC family members. This kinase has been reported to play roles in many different cellular processes, such as cell adhesion, cell transformation, cell cycle checkpoint, and cell volume control. Knockout studies in mice suggest that this kinase may be a fundamental regulator of cardiac contractility and Ca(2+) handling in myocytes.