AMPK Gamma 1 Polyclonal antibody

• Featured Product
• KD/KO Validated

AMPK Gamma 1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (2)

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

8

Cat no : 10290-1-AP

Synonyms

PRKAG1, AMPKG, AMPK subunit gamma-1, AMPK subunit gamma 1, AMPK gamma1

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Validation Data Gallery

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  WB analysis using 10290-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10290-1-AP (AMPK Gamma 1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 10290-1-AP

  WB result of AMPK Gamma 1 antibody (10290-1-AP; 1:5000; incubated at room temperature for 1.5 hours) with sh-Control and sh-AMPK Gamma 1 transfected HEK-293 cells.

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  IP experiment of K-562 using 10290-1-AP

  IP result of anti-AMPK Gamma 1 (IP:10290-1-AP, 4ug; Detection:10290-1-AP 1:1000) with K-562 cells lysate 3680 ug.

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  IHC staining of human testis using 10290-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 10290-1-AP (AMPK gamma 1 antibody at dilution of 1:50 (under 10x lens).

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  IHC staining of human testis using 10290-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 10290-1-AP (AMPK gamma 1 antibody at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human skeletal muscle using 10290-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 10290-1-AP (AMPK gamma 1 antibody at dilution of 1:50 (under 10x lens).

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  IHC staining of human skeletal muscle using 10290-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 10290-1-AP (AMPK gamma 1 antibody at dilution of 1:50 (under 40x lens).

• 

  IF Staining of HeLa using 10290-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 10290-1-AP (AMPK gamma 1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: HeLa cells, HEK-293 cells, Jurkat cells, K-562 cells
• Positive IP detected in: K-562 cells
• Positive IHC detected in: human testis tissue, human skeletal muscle tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 8 publications below

Product Information

10290-1-AP targets AMPK Gamma 1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: AMPK Gamma 1 fusion protein Ag0302
• Full Name: protein kinase, AMP-activated, gamma 1 non-catalytic subunit
• Calculated Molecular Weight: 38 kDa
• Observed Molecular Weight: 35-38 kDa
• GenBank Accession Number: BC000358
• Gene Symbol: AMPK Gamma 1
• Gene ID (NCBI): 5571
• RRID: AB_2268781
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Protein kinase, AMP-activated, gamma 1 non-catalytic subunit (PRKAG1, synonyms: AMPKG, MGC8666) is a regulatory subunit of the AMP-activated protein kinase (AMPK). AMPK is a heterotrimer consisting of an catalytic subunit, and non-catalytic and subunits. AMPK is an important energy-sensing enzyme that monitors cellular energy status. In response to cellular metabolic stresses, AMPK is activated, and thus phosphorylates and iAMP-activated protein kinase (AMPK) is a highly conserved heterotrimeric serine/threonine kinase widely characterised as a sensor of cellular energetic stress. AMPK is a heterotrimeric complex consisting of a catalytic α-subunit and two regulatory subunits (β and γ). AMPK is an important energy-sensing enzyme that monitors cellular energy status. In response to cellular metabolic stresses, AMPK is activated, and thus phosphorylates and inactivates acetyl-CoA carboxylase (ACC) and beta-hydroxy beta-methylglutaryl-CoA reductase (HMGCR), key enzymes involved in regulating de novo biosynthesis of fatty acid and cholesterol. AMPK gamma 1 is one of the gamma regulatory subunits of AMPK.

Publications

Species	Application	Title
human	WB	Cell Metab; CircACC1 Regulates Assembly and Activation of AMPK Complex under Metabolic Stress.; Authors - Qidong Li ; KD Validated
mouse	WB	JHEP Rep; Human umbilical cord mesenchymal stem cell-derived exosomes ameliorate liver steatosis by promoting fatty acid oxidation and reducing fatty acid synthesis; Authors - Fuji Yang
human	WB	J Med Chem; Identification of Pyruvate Carboxylase as the Cellular Target of Natural Bibenzyls with Potent Anticancer Activity against Hepatocellular Carcinoma via Metabolic Reprogramming.; Authors - Yuwen Sheng
human	WB	Protein Cell; AMPK interacts with DSCAM and plays an important role in netrin-1 induced neurite outgrowth.; Authors - Zhu Kun K
human	WB	J Exp Clin Cancer Res; METTL16 promotes glycolytic metabolism reprogramming and colorectal cancer progression; Authors - Wei Wei ; KD Validated
human	WB	Cell Death Dis; WW domain-binding protein 2 overexpression prevents diet-induced liver steatosis and insulin resistance through AMPKβ1.; Authors - Zhe Zheng