PRKACB Polyclonal antibody

PRKACB Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

21

Cat no : 55382-1-AP

Synonyms

PKA C beta, PKA C-beta-specific, PKACB, PRKACB

---

Validation Data Gallery

• 

  WB analysis of mouse brain using 55382-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 55382-1-AP (PKA C-beta-specifc antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 55382-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 55382-1-AP (PKA C-beta-specifc antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 55382-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 55382-1-AP (PKA C-beta-specifc antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 55382-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 55382-1-AP (PKA C-beta-specifc antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 55382-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 55382-1-AP (PKA C-beta-specifc antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human kidney using 55382-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney slide using 55382-1-AP (PKA C-beta-specifc antibody at dilution of 1:50.

• 

  IHC staining of human kidney using 55382-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney slide using 55382-1-AP (PKA C-beta-specifc antibody at dilution of 1:50.

---

Tested Applications

• Positive WB detected in: mouse brain tissue, human brain tissue
• Positive IHC detected in: human kidney tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 19 publications below
• IHC: See 3 publications below

Product Information

55382-1-AP targets PRKACB in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: protein kinase, cAMP-dependent, catalytic, beta
• Calculated Molecular Weight: 46 kDa
• Observed Molecular Weight: 36-55 kDa
• GenBank Accession Number: NM_002731
• Gene Symbol: PRKACB
• Gene ID (NCBI): 5567
• RRID: AB_10949078
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PRKACB, also named as PKA C-beta, belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family and cAMP subfamily. It mediates cAMP-dependent signaling triggered by receptor binding to GPCRs. PKA activation regulates diverse cellular processes such as cell proliferation, the cell cycle, differentiation and regulation of microtubule dynamics, chromatin condensation and decondensation, nuclear envelope disassembly and reassembly, as well as regulation of intracellular transport mechanisms and ion flux. This antibody is specific to PRKACB.

Publications

Species	Application	Title
human,mouse	WB	Environ Int; Exposure to dithiocarbamate fungicide maneb in vitro and in vivo: Neuronal apoptosis and underlying mechanisms; Authors - Chaoyang Liu
mouse	WB	Int J Mol Sci; Per1/Per2 Disruption Reduces Testosterone Synthesis and Impairs Fertility in Elderly Male Mice.; Authors - Qinrui Liu
human	WB	Oncogenesis; GSK3β palmitoylation mediated by ZDHHC4 promotes tumorigenicity of glioblastoma stem cells in temozolomide-resistant glioblastoma through the EZH2-STAT3 axis.; Authors - Chenggang Zhao
human	WB	Pharmacol Res; Hypericin attenuates nonalcoholic fatty liver disease and abnormal lipid metabolism via the PKA-mediated AMPK signaling pathway in vitro and in vivo.; Authors - Chen Liang
human	IHC	Am J Cancer Res; Different lipid metabolic profiles and their associated genes in sessile serrated adenoma or polyps compared to hyperplastic polyps.; Authors - Chaotao Tang
human	WB	Viruses; ICP4-Associated Activation of Rap1b Facilitates Herpes Simplex Virus Type I (HSV-1) Infection in Human Corneal Epithelial Cells; Authors - Beibei Zhang