PRKACB Polyclonal antibody, PBS Only

PRKACB Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Conjugate

Unconjugated

Cat no : 12232-1-PBS

Synonyms

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Validation Data Gallery

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  WB analysis of MCF-7 using 12232-1-AP (same clone as 12232-1-PBS)

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 12232-1-AP (PRKACB Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12232-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse heart using 12232-1-AP (same clone as 12232-1-PBS)

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 12232-1-AP (PRKACB Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12232-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HT-1080 using 12232-1-AP (same clone as 12232-1-PBS)

  HT-1080 cells were subjected to SDS PAGE followed by western blot with 12232-1-AP (PRKACB antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12232-1-PBS in a different storage buffer formulation.

• 

  WB analysis of A549 using 12232-1-AP (same clone as 12232-1-PBS)

  A549 cells were subjected to SDS PAGE followed by western blot with 12232-1-AP (PRKACB antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12232-1-PBS in a different storage buffer formulation.

• 

  WB analysis of PC-3 using 12232-1-AP (same clone as 12232-1-PBS)

  PC-3 cells were subjected to SDS PAGE followed by western blot with 12232-1-AP (PRKACB antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12232-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse brain using 12232-1-AP (same clone as 12232-1-PBS)

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 12232-1-AP (PRKACB antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12232-1-PBS in a different storage buffer formulation.

• 

  WB analysis of MCF-7 using 12232-1-AP (same clone as 12232-1-PBS)

  MCF7 cells were subjected to SDS PAGE followed by western blot with 12232-1-AP (PRKACB antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12232-1-PBS in a different storage buffer formulation.

• 

  IP experiment of mouse brain using 12232-1-AP (same clone as 12232-1-PBS)

  IP result of anti-PRKACB (IP:12232-1-AP, 4ug; Detection:12232-1-AP 1:500) with mouse brain tissue lysate 2640ug. This data was developed using the same antibody clone with 12232-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brain using 12232-1-AP (same clone as 12232-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12232-1-AP (PRKACB antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12232-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brain using 12232-1-AP (same clone as 12232-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12232-1-AP (PRKACB antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12232-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human medulloblastoma using 12232-1-AP (same clone as 12232-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human medulloblastoma using 12232-1-AP (PRKACB antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 12232-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HeLa using 12232-1-AP (same clone as 12232-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using PRKACB antibody (12232-1-AP) at dilution of 1:200 and CoraLite®594-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 12232-1-PBS in a different storage buffer formulation.

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Product Information

12232-1-PBS targets PRKACB in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PRKACB fusion protein Ag2870
• Full Name: protein kinase, cAMP-dependent, catalytic, beta
• Calculated Molecular Weight: 41 kDa, 46 kDa
• Observed Molecular Weight: 36-55 kDa
• GenBank Accession Number: BC016285
• Gene Symbol: PRKACB
• Gene ID (NCBI): 5567
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

PRKACB, also named as PKA C-beta, belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family and cAMP subfamily. It mediates cAMP-dependent signaling triggered by receptor binding to GPCRs. PKA activation regulates diverse cellular processes such as cell proliferation, the cell cycle, differentiation and regulation of microtubule dynamics, chromatin condensation and decondensation, nuclear envelope disassembly and reassembly, as well as regulation of intracellular transport mechanisms and ion flux.