PRKACA Polyclonal antibody

• Featured Product
• KD/KO Validated

PRKACA Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (2)

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

14

Cat no : 27398-1-AP

Synonyms

PKA C alpha, PKACA, PRKACA

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Validation Data Gallery

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  WB analysis using 27398-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 27398-1-AP (PRKACA antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 27398-1-AP

  WB result of PRKACA antibody (27398-1-AP; 1:300; incubated at room temperature for 1.5 hours) with sh-Control and sh-PRKACA transfected HeLa cells.

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  IP experiment of HeLa using 27398-1-AP

  IP result of anti-PRKACA (IP:27398-1-AP, 4ug; Detection:27398-1-AP 1:300) with HeLa cells lysate 2600 ug.

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  IHC staining of human testis using 27398-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 27398-1-AP (PRKACA antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human testis using 27398-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 27398-1-AP (PRKACA antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human heart using 27398-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 27398-1-AP (PRKACA antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human heart using 27398-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 27398-1-AP (PRKACA antibody) at dilution of 1:200 (under 40x lens).

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  IF Staining of U2OS using 27398-1-AP

  Immunofluorescent analysis of (4% PFA) fixed U2OS cells using PRKACA antibody (27398-1-AP) at dilution of 1:200 and Cy3-conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: HeLa cells, Neuro-2a cells, SH-SY5Y cells
• Positive IP detected in: HeLa cells
• Positive IHC detected in: human testis tissue, human heart tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: U2OS cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 13 publications below
• IP: See 1 publications below

Product Information

27398-1-AP targets PRKACA in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse, rat, chicken
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PRKACA fusion protein Ag26392
• Full Name: protein kinase, cAMP-dependent, catalytic, alpha
• Calculated Molecular Weight: 41 kDa
• Observed Molecular Weight: 38-43 kDa
• GenBank Accession Number: BC039846
• Gene Symbol: PRKACA
• Gene ID (NCBI): 5566
• RRID: AB_2880861
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
rat	WB	Front Pharmacol; Phosphoproteomic and proteomic profiling in post-infarction chronic heart failure; Authors - Jiayue Wang
chicken	WB	Microbiol Spectr; Avian Hepatitis E Virus ORF2 Protein Interacts with Rap1b to Induce Cytoskeleton Rearrangement That Facilitates Virus Internalization.; Authors - Beibei Zhang
human,mouse	WB,IP	Eur J Pharmacol; Theaflavine inhibits hepatic stellate cell activation by modulating the PKA/LKB1/AMPK/GSK3β cascade and subsequently enhancing Nrf2 signaling; Authors - Guangwen Shu
human	WB	Mediators Inflamm; Vitronectin Destroyed Intestinal Epithelial Cell Differentiation through Activation of PDE4-Mediated Ferroptosis in Inflammatory Bowel Disease; Authors - Wenxu Pan
human	WB	Cell Rep; Protein modifications throughout the lung cancer proteome unravel the cancer-specific regulation of glycolysis.; Authors - Yangmiao Duan ; KD Validated
mouse	WB	Physiol Behav; Protection of p-Coumaric acid against chronic stress-induced neurobehavioral deficits in mice via activating the PKA-CREB-BDNF pathway; Authors - Yanqun Cao