AMPK Beta 2 Polyclonal antibody

AMPK Beta 2 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 14429-1-AP

Synonyms

PRKAB2, AMPK subunit beta-2, AMPK subunit beta 2, AMPK, 5'-AMP-activated protein kinase subunit beta-2

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Validation Data Gallery

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  WB analysis using 14429-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14429-1-AP (AMPK beta 2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of fetal human brain using 14429-1-AP

  fetal human brain tissue were subjected to SDS PAGE followed by western blot with 14429-1-AP (AMPK beta 2 antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human heart using 14429-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 14429-1-AP (AMPK beta 2 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 14429-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 14429-1-AP (AMPK beta 2 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human colon using 14429-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 14429-1-AP (AMPK Beta 2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon using 14429-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 14429-1-AP (AMPK Beta 2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human kidney using 14429-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 14429-1-AP (AMPK Beta 2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human kidney using 14429-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 14429-1-AP (AMPK Beta 2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 14429-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using AMPK Beta 2 antibody (14429-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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Tested Applications

• Positive WB detected in: HEK-293 cells, human heart tissue, human brain tissue, fetal human brain tissue, mouse testis tissue, mouse brain tissue, rat brain tissue
• Positive IHC detected in: human colon tissue, human kidney tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

14429-1-AP targets AMPK Beta 2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: AMPK Beta 2 fusion protein Ag5806
• Full Name: protein kinase, AMP-activated, beta 2 non-catalytic subunit
• Calculated Molecular Weight: 30 kDa
• Observed Molecular Weight: 34 kDa
• GenBank Accession Number: BC053610
• Gene Symbol: AMPK Beta 2
• Gene ID (NCBI): 5565
• RRID: AB_2252925
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.