PRIM2 Polyclonal antibody

PRIM2 Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 11788-1-AP

Synonyms

PRIM2A, p58

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Validation Data Gallery

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  WB analysis using 11788-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11788-1-AP (PRIM2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  IF Staining of A375 using 11788-1-AP

  Immunofluorescent analysis of (4% PFA) fixed A375 cells using 11788-1-AP (PRIM2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: A375 cells, HeLa cells, MCF-7 cells, NIH/3T3 cells
• Positive IF/ICC detected in: A375 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

11788-1-AP targets PRIM2 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PRIM2 fusion protein Ag2370
• Full Name: primase, DNA, polypeptide 2 (58kDa)
• Calculated Molecular Weight: 58 kDa
• Observed Molecular Weight: 58 kDa
• GenBank Accession Number: BC017833
• Gene Symbol: PRIM2
• Gene ID (NCBI): 5558
• RRID: AB_2169380
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.