PRICKLE3 Polyclonal antibody

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  WB analysis of Jurkat using 19098-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 19098-1-AP (PRICKLE3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 19098-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 19098-1-AP (PRICKLE3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 19098-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 19098-1-AP (PRICKLE3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of human colon using 19098-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon using 19098-1-AP (PRICKLE3 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human colon using 19098-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon using 19098-1-AP (PRICKLE3 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human heart using 19098-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 19098-1-AP (PRICKLE3 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human heart using 19098-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 19098-1-AP (PRICKLE3 antibody) at dilution of 1:50 (under 40x lens).

PRICKLE3 Polyclonal Antibody for WB, IHC, ELISA

Cat No. 19098-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Synonyms

LIM domain only protein 6, LMO 6, LMO6, prickle homolog 3 (Drosophila), Prickle like protein 3, PRICKLE3, Triple LIM domain protein 6

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Tested Applications

• Positive WB detected in: Jurkat cells, HeLa cells
• Positive IHC detected in: human colon tissue, human heart tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

19098-1-AP targets PRICKLE3 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PRICKLE3 fusion protein Ag13632
• Full Name: prickle homolog 3 (Drosophila)
• Calculated Molecular Weight: 69 kDa
• Observed Molecular Weight: 69 kDa
• GenBank Accession Number: BC016856
• Gene Symbol: PRICKLE3
• Gene ID (NCBI): 4007
• RRID: AB_10644341
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: O43900
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.