PRG2 Polyclonal antibody

PRG2 Polyclonal Antibody for IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF, IHC, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 10766-1-AP

Synonyms

BMPG, Bone marrow proteoglycan, EMBP, MBP, MBP1, PRG2, Proteoglycan 2

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Validation Data Gallery

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  IHC staining of human breast cancer using 10766-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10766-1-AP (PRG2 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 10766-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10766-1-AP (PRG2 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 10766-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10766-1-AP (PRG2 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 10766-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10766-1-AP (PRG2 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive IHC detected in: human breast cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• IHC: See 1 publications below
• IF: See 1 publications below

Product Information

10766-1-AP targets PRG2 in IF, IHC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PRG2 fusion protein Ag1150
• Full Name: proteoglycan 2, bone marrow (natural killer cell activator, eosinophil granule major basic protein)
• Calculated Molecular Weight: 25 kDa
• GenBank Accession Number: BC005929
• Gene Symbol: PRG2
• Gene ID (NCBI): 5553
• RRID: AB_2169223
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PRG2, also known as BMPG, has 222 amino acid residues with a 16 N-terminal signal sequence, so the mature form has 206 amino acid residues. High levels of PRG2 are present in placenta and pregnancy serum, where it exists as a complex with several other proteins including pregnancy-associated plasma protein A (PAPPA), angiotensinogen (AGT), and C3dg. This protein can be cleaved into eosinophil granule major basic protein (MBP), which is the major constituent of the crystalline core of the eosinophil granule. MBP is highly basic and toxic to both mammalian cells and parasites, and it functions in the defense against invading parasites (PMID: 21270431).

Publications

Species	Application	Title
human	IF	Allergy; Immunoglobulin G4 in eosinophilic esophagitis: Immune complex formation and correlation with disease activity; Authors - Jonathan G Medernach
human	IHC	Exp Eye Res; Role of TGF-β in tissue eosinophilia associated with vernal keratoconjunctivitis.; Authors - Ohtomo Kaori K