PRDX3 Recombinant antibody, PBS Only

• Featured Product
• KD/KO Validated

PRDX3 Recombinant Antibody for WB, IF, IHC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, Mouse, Rat

Applications

WB, IF, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1L11

Cat no : 81833-1-PBS

Synonyms

Antioxidant protein 1, AOP 1, AOP1, HBC189, MER5, peroxiredoxin 3, Peroxiredoxin III, PRDX3, PRO1748, Protein MER5 homolog, Prx III, PRX3, SP 22

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Validation Data Gallery

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  WB analysis using 81833-1-RR (same clone as 81833-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 81833-1-RR (PRX3 antibody) at dilution of 1:40000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 81833-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HEK-293 using 81833-1-RR (same clone as 81833-1-PBS)

  WB result of PRDX3 antibody (81833-1-RR; 1:100000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PRDX3 transfected HEK-293 cells. This data was developed using the same antibody clone with 81833-1-PBS in a different storage buffer formulation.

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  IHC staining of human lung cancer using 81833-1-RR (same clone as 81833-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 81833-1-RR (PRDX3 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81833-1-PBS in a different storage buffer formulation.

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  IHC staining of human lung cancer using 81833-1-RR (same clone as 81833-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 81833-1-RR (PRDX3 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81833-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 81833-1-RR (same clone as 81833-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using PRDX3 antibody (81833-1-RR, Clone: 1L11 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 81833-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HepG2 using 81833-1-RR (same clone as 81833-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using PRX3 antibody (81833-1-RR, Clone: 1L11 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 81833-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HepG2 using 81833-1-RR (same clone as 81833-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed HepG2 cells using PRDX3 antibody (81833-1-RR, Clone: 1L11 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 81833-1-PBS in a different storage buffer formulation.

• 

  Affinity experiments of 81833-1-RR

  Biolayer interferometry (BLl) kinetic assays of 81833-1-RR against Human PRDX3 were performed. The affinity constant is below 1 pM.

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Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

81833-1-PBS targets PRDX3 in WB, IF, IHC, Indirect ELISA applications and shows reactivity with Human, Mouse, Rat samples.

• Tested Reactivity: Human, Mouse, Rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: PRDX3 fusion protein Ag1062
• Full Name: peroxiredoxin 3
• Calculated Molecular Weight: 27 kDa
• Observed Molecular Weight: 28 kDa
• GenBank Accession Number: BC007062
• Gene Symbol: PRDX3
• Gene ID (NCBI): 10935
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

PRDX3(Peroxiredoxin 3), also named as AOP1, HBC189 and MER5, belongs to the ahpC/TSA family. It is involved in redox regulation of the cell and protects radical-sensitive enzymes from oxidative damage by a radical-generating system. PRDX3 is required for MYC-mediated proliferation, transformation, and apoptosis after glucose withdrawal and essential for maintaining mitochondrial mass and membrane potential in transformed rat and human cells (PMID:12011429). PRDX3 acts synergistically with MAP3K13 to regulate the activation of NF-kappa-B in the cytosol.