peroxiredoxin 2 Polyclonal antibody

• Featured Product
• KD/KO Validated

peroxiredoxin 2 Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 20569-1-AP

Synonyms

PRDX2, Peroxiredoxin-2, NKEF-B, NKEFB, Natural killer cell-enhancing factor B

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Validation Data Gallery

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  WB analysis of HeLa using 20569-1-AP

  WB result of peroxiredoxin 2 antibody (20569-1-AP, 1:5000) with si-Control and si-peroxiredoxin 2 transfected HeLa cells.

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  WB analysis of HeLa using 20569-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 20569-1-AP (peroxiredoxin 2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 20569-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 20569-1-AP (peroxiredoxin 2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IF Staining of HepG2 using 20569-1-AP

  Immunofluorescent analysis of HepG2 cells, using PRDX2 antibody 20569-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Tested Applications

• Positive WB detected in: HeLa cells
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

20569-1-AP targets peroxiredoxin 2 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: peroxiredoxin 2
• Calculated Molecular Weight: 22 kDa
• Observed Molecular Weight: 22 kDa
• GenBank Accession Number: NM_005809
• Gene Symbol: peroxiredoxin 2
• Gene ID (NCBI): 7001
• RRID: AB_10695186
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PRDX2, also named as NKEFB, TDPX1, TSA and PRP, belongs to the ahpC/TSA family. It is involved in redox regulation of the cell. Reduces peroxides with reducing equivalents provided through the thioredoxin system. It is not able to receive electrons from glutaredoxin. PRDX2 may play an important role in eliminating peroxides generated during metabolism. It might participate in the signaling cascades of growth factors and tumor necrosis factor-alpha by regulating the intracellular concentrations of H2O2. The antibody is specific to PRDX2.