PRDX1 Polyclonal antibody

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• KD/KO Validated

PRDX1 Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 20568-1-AP

Synonyms

PAGA, PAG, NKEF-A, Natural killer cell-enhancing factor A, MSP23

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Validation Data Gallery

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  WB analysis using 20568-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 20568-1-AP (PRDX1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 20568-1-AP

  WB result of PRDX1 antibody (20568-1-AP; 1:6000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PRDX1 transfected HEK-293 cells.

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  IF Staining of HepG2 using 20568-1-AP

  Immunofluorescent analysis of HepG2 cells, using PRDX1 antibody 20568-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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  IF Staining of HeLa using 20568-1-AP

  Immunofluorescent analysis of Hela cells, using PRDX1 antibody 10568-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Tested Applications

• Positive WB detected in: HEK-293 cells, HeLa cells, Jurkat cells, MCF-7 cells
• Positive IF/ICC detected in: HepG2 cells, Hela cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:8000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

20568-1-AP targets PRDX1 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: peroxiredoxin 1
• Calculated Molecular Weight: 22 kDa
• Observed Molecular Weight: 23 kDa
• GenBank Accession Number: NM_002574
• Gene Symbol: PRDX1
• Gene ID (NCBI): 5052
• RRID: AB_10693622
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PRDX1(Peroxiredoxin-1), also named as PAGA, PAGB, TDPX2, PAG or NKEF-A, belongs to the ahpC/TSA family. PRDX1 is a thiol reductase that plays critical roles in oxidative and thermal stress defense mechanisms through its abilities to metabolize H2O2 and act as a molecular chaperone, respectively. PRDX1 might participate in the signaling cascades of growth factors and tumor necrosis factor-alpha by regulating the intracellular concentrations of H2O2 ((PMID: 9497357)). It reduces an intramolecular disulfide bond in GDPD5 that gates the ability to GDPD5 to drive postmitotic motor neuron differentiation. PRDX1 can form a dimer, and also can be phosphorylated on Thr-90 during the M-phase, which leads to a more than 80% decrease in enzymatic activity (PMID: 22583657, 11986303). The antibody is specific to PRDX1.

Publications

Species	Application	Title
		Cell Chem Biol; Parvifoline AA Promotes Susceptibility of Hepatocarcinoma to Natural Killer Cell-Mediated Cytolysis by Targeting Peroxiredoxin.; Authors - Huifang Zhu