PRDM16 Recombinant antibody, PBS Only

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  WB analysis using 83872-1-RR (same clone as 83872-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 83872-1-RR (PRDM16 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83872-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brown adipose using 83872-1-RR (same clone as 83872-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brown adipose tissue slide using 83872-1-RR (PRDM16 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83872-1-PBS in a different storage buffer formulation.

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  IF Staining of HEK-293 using 83872-1-RR (same clone as 83872-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using PRDM16 antibody (83872-1-RR, Clone: 240662D3 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83872-1-PBS in a different storage buffer formulation.

PRDM16 {ptg:Brand} Recombinant Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 83872-1-PBS

Clone No.240662D3

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

MDS1/EVI1-like gene 1, MDS1/EVI1 like gene 1, KIAA1675, Histone-lysine N-methyltransferase PRDM16, EC:2.1.1.367

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Product Information

83872-1-PBS targets PRDM16 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Peptide
• Full Name: PR domain containing 16
• Calculated Molecular Weight: 140 kDa
• Observed Molecular Weight: ~140 kDa
• GenBank Accession Number: NM_022114
• Gene Symbol: PRDM16
• Gene ID (NCBI): 63976
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: Q9HAZ2
• Storage Buffer: PBS only , pH 7.3
• Storage Conditions: Store at -80°C.

Background Information

PR domain containing 16, also known as PRDM16, acts as a transcription coregulator that controls the development of brown adipocytes in brown adipose tissue. Previously, this coregulator was believed to be present only in brown adipose tissue, but more recent studies have shown that PRDM16 is highly expressed in subcutaneous white adipose tissue as well. The protein encoded by this gene is a zinc finger transcription factor. PRDM16 controls the cell fate between muscle and brown fat cells. Loss of PRDM16 from brown fat precursors causes a loss of brown fat characteristics and promotes muscle differentiation.