PRDM16 Polyclonal antibody

PRDM16 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 55361-1-AP

Synonyms

MEL1, MDS1/EVI1-like gene 1, MDS1/EVI1 like gene 1, KIAA1675, Histone-lysine N-methyltransferase PRDM16

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Validation Data Gallery

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  WB analysis using 55361-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 55361-1-AP (PRDM16 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  IHC staining of human stomach using 55361-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 55361-1-AP (PRDM16 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: Jurkat cells, K-562 cells, NCI-H1299 cells
• Positive IHC detected in: human stomach tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

PRDM16 has low expression in most cells and tissues

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

55361-1-AP targets PRDM16 in WB, IHC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: PR domain containing 16
• Calculated Molecular Weight: 140 kDa
• Observed Molecular Weight: ~150 kDa
• GenBank Accession Number: NM_022114
• Gene Symbol: PRDM16
• Gene ID (NCBI): 63976
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.