PRC1 Polyclonal antibody

• Featured Product
• KD/KO Validated

PRC1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 10110-2-AP

Synonyms

ASE1, PRC1

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Validation Data Gallery

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  WB analysis of MCF-7 using 10110-2-AP

  WB result of PRC1 antibody (10110-2-AP; 1:6000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PRC1 transfected MCF-7 cells.

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  WB analysis using 10110-2-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10110-2-AP (PRC1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IHC staining of human colon cancer using 10110-2-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10110-2-AP (PRC1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 10110-2-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10110-2-AP (PRC1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: MCF-7 cells, HEK-293 cells, HeLa cells, HepG2 cells
• Positive IHC detected in: human colon cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:12000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

10110-2-AP targets PRC1 in WB, IHC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PRC1 fusion protein Ag0163
• Full Name: protein regulator of cytokinesis 1
• Calculated Molecular Weight: 67 kDa
• Observed Molecular Weight: 67 kDa
• GenBank Accession Number: BC005140
• Gene Symbol: PRC1
• Gene ID (NCBI): 9055
• RRID: AB_2252888
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PRC1 is involved in cytokinesis. PRC1 is at high level during S and G2/M and drop dramatically after cell exit mitosis and enter G1. PRC1 is located in the nucleus during interphase, and becomes associated with mitotic spindles in a highly dynamic manner during mitosis, and localizes to the cell mid-body during cytokinesis. PRC1 is a good substrate for several cyclin-dependent kinases (CDKs) in vitro and is phosphorylated in vivo at sites that are phosphorylated by CDK in vitro, suggesting that PRC1 is an in vivo CDK substrate.

Publications

Species	Application	Title
human	WB	Immun Inflamm Dis; E2F3 renders an immunosuppressive tumor microenvironment in nasopharyngeal carcinoma: Involvements of the transcription activation of PRC1 and BIRC5; Authors - Qiang Wang