PR Polyclonal antibody

PR Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (2)

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

21

Cat no : 25871-1-AP

Synonyms

PGR, PRGR, NR3C3

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Validation Data Gallery

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  WB analysis of T-47D using 25871-1-AP

  T-47D cells were subjected to SDS PAGE followed by western blot with 25871-1-AP (PR antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human breast cancer using 25871-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 25871-1-AP (PR antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 25871-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 25871-1-AP (PR antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human ovary tumor using 25871-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 25871-1-AP (PR antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human ovary tumor using 25871-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 25871-1-AP (PR antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of T-47D using 25871-1-AP

  Immunofluorescent analysis of (4% PFA) fixed T-47D cells using PR antibody (25871-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: T-47D cells
• Positive IHC detected in: human breast cancer tissue, human ovary tumor tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: T-47D cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochemistry (IHC): IHC : 1:200-1:800
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 8 publications below
• IHC: See 14 publications below
• IF: See 1 publications below

Product Information

25871-1-AP targets PR in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse, rat, goat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PR fusion protein Ag22986
• Full Name: progesterone receptor
• Calculated Molecular Weight: 933 aa, 99 kDa
• Observed Molecular Weight: 80 kDa, 110-115 kDa
• GenBank Accession Number: NM_000926
• Gene Symbol: Progesterone Receptor
• Gene ID (NCBI): 5241
• RRID: AB_2880277
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PGR, also named as NR3C3 and PR, belongs to the nuclear hormone receptor family and NR3 subfamily. The steroid hormones and their receptors are involved in the regulation of eukaryotic gene expression and affect cellular proliferation and differentiation in target tissues. Progesterone receptor isoform B (PRB) is involved activation of c-SRC/MAPK signaling on hormone stimulation. The ER and PR status has been used as a predictor of breast carcinoma responsiveness to endocrine therapy and as a prognostic indicator for early recurrence.

Publications

Species	Application	Title
human	WB	Nat Commun; Glucocorticoid receptor regulates PD-L1 and MHC-I in pancreatic cancer cells to promote immune evasion and immunotherapy resistance.; Authors - Yalan Deng
human	IF,WB	Nucleic Acids Res; ZNF598 co-translationally titrates poly(GR) protein implicated in the pathogenesis of C9ORF72-associated ALS/FTD; Authors - Jumin Park
human	IHC	Acta Neuropathol; Increased prevalence of granulovacuolar degeneration in C9orf72 mutation.; Authors - Yuichi Riku
goat	WB,IHC	Int J Mol Sci; The High Level of RANKL Improves IκB/p65/Cyclin D1 Expression and Decreases p-Stat5 Expression in Firm Udder of Dairy Goats; Authors - Zhen Gao
rat	WB,IHC	Front Endocrinol (Lausanne); Effect of acupuncture on the opening time of implantation window and endometrial receptivity in controlled ovarian hyperstimulation rats during peri-implantation period; Authors - Runan Hu
human	IHC	Front Oncol; Pan-Cancer Prognostic Role and Targeting Potential of the Estrogen-Progesterone Axis.; Authors - Yu-Ting Shen