PQBP1 Recombinant antibody

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  WB analysis using 84491-6-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 84491-6-RR (PQBP1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  IF Staining of HeLa using 84491-6-RR

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using PQBP1 antibody (84491-6-RR, Clone: 241901B11 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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  Affinity and Kinetic Characterization of 84491-6-RR

  Biolayer interferometry (BLl) kinetic assays of 84491-6-RR against Human PQBP1 were performed. The affinity constant is 0.529 nM.

PQBP1 Recombinant Antibody for WB, IF/ICC, ELISA

Cat No. 84491-6-RR

Clone No.241901B11

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Synonyms

PQBP-1, Polyglutamine-binding protein 1, Polyglutamine tract-binding protein 1, Npw38, 241901B11

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Tested Applications

• Positive WB detected in: HeLa cells, HEK-293T cells, mouse pancreas tissue, rat brain tissue, rat thymus tissue
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:8000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

84491-6-RR targets PQBP1 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: PQBP1 fusion protein Ag9329
• Full Name: polyglutamine binding protein 1
• Calculated Molecular Weight: 265 aa, 30 kDa
• Observed Molecular Weight: 30-32 kDa
• GenBank Accession Number: BC012358
• Gene Symbol: PQBP1
• Gene ID (NCBI): 10084
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: O60828
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PQBP1 (Polyglutamine-binding protein 1) is also named as NPW38 and JM26. PQBP1 senses extrinsic tau 3R/4R proteins by direct interaction and triggers an innate immune response by activating a cyclic GMP-AMP synthase (cGAS)-Stimulator of interferon genes (STING) pathway (PMID: 34782623).  PQBP1 is abundantly expressed in the central nervous system during development. PQBP1 is a component of the precatalytic spliceosome (B complex) that is involved in splicing. PQBP1 was predominantly localized in the cell nucleus. PQBP1 is overexpressed in various cancer types including breast cancer, colon cancer, and glioblastoma (PMID: 38342602).