PQBP1 Polyclonal antibody

PQBP1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

4

Cat no : 16264-1-AP

Synonyms

PQBP-1, Polyglutamine-binding protein 1, Polyglutamine tract-binding protein 1, Npw38

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Validation Data Gallery

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  WB analysis using 16264-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 16264-1-AP (PQBP1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse skeletal muscle using 16264-1-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 16264-1-AP (PQBP1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 16264-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 16264-1-AP (PQBP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 16264-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 16264-1-AP (PQBP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 16264-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 16264-1-AP (PQBP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat brain using 16264-1-AP

  rat brain tissue were subjected to SDS PAGE followed by western blot with 16264-1-AP (PQBP1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse brain using 16264-1-AP

  IP result of anti-PQBP1 (IP:16264-1-AP, 4ug; Detection:16264-1-AP 1:500) with mouse brain tissue lysate 3200 ug.

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  IHC staining of human breast cancer using 16264-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 16264-1-AP (PQBP1 antibody) at dilution of 1:1000 (under 40x lens).

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  IHC staining of human colon using 16264-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 16264-1-AP (PQBP1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat colon using 16264-1-AP

  Immunohistochemical analysis of paraffin-embedded rat colon tissue slide using 16264-1-AP (PQBP1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 16264-1-AP

  Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 16264-1-AP (PQBP1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: HeLa cells, mouse brain tissue, mouse skeletal muscle tissue, rat brain tissue
• Positive IP detected in: mouse brain tissue
• Positive IHC detected in: human breast cancer tissue, human colon tissue, rat colon tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:500-1:2000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 3 publications below
• IHC: See 1 publications below
• IF: See 2 publications below
• IP: See 2 publications below

Product Information

16264-1-AP targets PQBP1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PQBP1 fusion protein Ag9329
• Full Name: polyglutamine binding protein 1
• Calculated Molecular Weight: 265 aa, 30 kDa
• Observed Molecular Weight: 30-32 kDa
• GenBank Accession Number: BC012358
• Gene Symbol: PQBP1
• Gene ID (NCBI): 10084
• RRID: AB_10792928
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PQBP1 (Polyglutamine-binding protein 1) is also named as NPW38 and JM26. PQBP1 senses extrinsic tau 3R/4R proteins by direct interaction and triggers an innate immune response by activating a cyclic GMP-AMP synthase (cGAS)-Stimulator of interferon genes (STING) pathway (PMID: 34782623).  PQBP1 is abundantly expressed in the central nervous system during development. PQBP1 is a component of the precatalytic spliceosome (B complex) that is involved in splicing. PQBP1 was predominantly localized in the cell nucleus. PQBP1 is overexpressed in various cancer types including breast cancer, colon cancer, and glioblastoma (PMID: 38342602).

Publications

Species	Application	Title
human	WB, IP, IF	Mol Cell; PQBP1 promotes translational elongation and regulates hippocampal mGluR-LTD by suppressing eEF2 phosphorylation.; Authors - Yuqian Shen
human	IHC	Acta Neuropathol; Transportin 1 accumulates specifically with FET proteins but no other transportin cargos in FTLD-FUS and is absent in FUS inclusions in ALS with FUS mutations.; Authors - Neumann Manuela M
human,mouse	WB,IP,IF	Cell Rep; Dynamic regulation of alternative polyadenylation by PQBP1 during neurogenesis; Authors - Xian Liu ; KD Validated
human	WB	mBio; Barrier-to-Autointegration Factor 1 Protects against a Basal cGAS-STING Response.; Authors - Hongming Ma