PPWD1 Polyclonal antibody

PPWD1 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 17106-1-AP

Synonyms

KIAA0073, PPWD1

---

Validation Data Gallery

• 

  WB analysis of Sp2/0 using 17106-1-AP

  Sp2/0 cells were subjected to SDS PAGE followed by western blot with 17106-1-AP (PPWD1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Sp2/0 using 17106-1-AP

  Sp2/0 cells were subjected to SDS PAGE followed by western blot with 17106-1-AP (PPWD1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse kidney using 17106-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 17106-1-AP (PPWD1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse testis using 17106-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 17106-1-AP (PPWD1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 17106-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 17106-1-AP (PPWD1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 17106-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 17106-1-AP (PPWD1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 17106-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 17106-1-AP (PPWD1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 17106-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 17106-1-AP (PPWD1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

---

Tested Applications

• Positive WB detected in: Sp2/0 cells, HEK-293 cells, HeLa cells, Jurkat cells, mouse brain tissue, mouse kidney tissue, mouse liver tissue, mouse testis tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

17106-1-AP targets PPWD1 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PPWD1 fusion protein Ag10785
• Full Name: peptidylprolyl isomerase domain and WD repeat containing 1
• Calculated Molecular Weight: 646 aa, 74 kDa
• Observed Molecular Weight: 65-66 kDa
• GenBank Accession Number: BC015385
• Gene Symbol: PPWD1
• Gene ID (NCBI): 23398
• RRID: AB_2237509
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Peptidylprolyl isomerase domain and WD repeat-containing protein1 belongs to cyclophilin family.PPWD1 accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides. May be involved in pre-mRNA splicing.This antibody recognizes the C-terminal of PPWD1.