PPT1 Monoclonal antibody, PBS Only

• Featured Product
• KD/KO Validated

PPT1 Monoclonal Antibody for WB, Indirect ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human, Pig, Rabbit

Applications

WB, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1A1A3

Cat no : 67699-1-PBS

Synonyms

CLN1, INCL, Palmitoyl protein hydrolase 1, PPT, PPT 1, PPT1

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Validation Data Gallery

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  WB analysis using 67699-1-Ig (same clone as 67699-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67699-1-Ig (PPT1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67699-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 67699-1-Ig (same clone as 67699-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67699-1-Ig (PPT1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67699-1-PBS in a different storage buffer formulation.

• 

  WB analysis of A549 using 67699-1-Ig (same clone as 67699-1-PBS)

  WB result of PPT1 antibody (67699-1-Ig; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PPT1 transfected A549 cells. This data was developed using the same antibody clone with 67699-1-PBS in a different storage buffer formulation.

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  WB analysis of pig heart using 67699-1-Ig (same clone as 67699-1-PBS)

  pig heart tissue were subjected to SDS PAGE followed by western blot with 67699-1-Ig (PPT1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67699-1-PBS in a different storage buffer formulation.

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Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67699-1-PBS targets PPT1 in WB, Indirect ELISA applications and shows reactivity with Human, Pig, Rabbit samples.

• Tested Reactivity: Human, Pig, Rabbit
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: PPT1 fusion protein Ag30550
• Full Name: palmitoyl-protein thioesterase 1
• Calculated Molecular Weight: 34 kDa
• Observed Molecular Weight: 32-34 kda
• GenBank Accession Number: BC008426
• Gene Symbol: PPT1
• Gene ID (NCBI): 5538
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

The defective gene behind the INCL(infantile neuronal ceroid lipofuscinoses) disease, CLN1, encodes for palmitoyl protein thioesterase 1 (PPT1). It consists of 306 amino acids, including a signal sequence of 26 amino acids and three N-linked glycosylation sites. This protein exists with a molecular mass of 32 kDa, 34 kDa,36 kDa, and 38 kDa. The enzyme is transported into lysosomes of non-neuronal cells by the mannose 6-phosphate receptor (M6PR) mediated pathway and a significant amount of native PPT1 resides in a complex rather than in a monomeric form(PMID:17565660).