PPP2R5D Polyclonal antibody

PPP2R5D Polyclonal Antibody for WB, IHC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IP, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 12068-1-AP

Synonyms

PP2A B subunit isoform R5-delta, PP2A B subunit isoform PR61-delta, PP2A B subunit isoform B'-delta, PP2A B subunit isoform B56-delta, PP2A B subunit isoform B delta

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Validation Data Gallery

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  WB analysis of Y79 using 12068-1-AP

  Y79 cells were subjected to SDS PAGE followed by western blot with 12068-1-AP (PPP2R5D antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 12068-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 12068-1-AP (PPP2R5D antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  IP experiment of Y79 using 12068-1-AP

  IP result of anti-PPP2R5D (IP:12068-1-AP, 4ug; Detection:12068-1-AP 1:400) with Y79 cells lysate 2000ug.

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  IHC staining of human stomach using 12068-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 12068-1-AP (PPP2R5D antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human stomach using 12068-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 12068-1-AP (PPP2R5D antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: Y79 cells, human brain tissue
• Positive IP detected in: Y79 cells
• Positive IHC detected in: human stomach tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 2 publications below

Product Information

12068-1-AP targets PPP2R5D in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PPP2R5D fusion protein Ag2705
• Full Name: protein phosphatase 2, regulatory subunit B', delta isoform
• Calculated Molecular Weight: 602 aa, 70 kDa
• Observed Molecular Weight: 70 kDa
• GenBank Accession Number: BC010692
• Gene Symbol: PPP2R5D
• Gene ID (NCBI): 5528
• RRID: AB_2299977
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB	EMBO J; The CEP170B-KIF2A complex destabilizes microtubule minus ends to generate polarized microtubule network; Authors - Cuirong Guan
mouse	WB	Biomedicines; Unmasking Protein Phosphatase 2A Regulatory Subunit B as a Crucial Factor in the Progression of Dilated Cardiomyopathy; Authors - Fang Lin ; KD Validated