PPP2R5A Polyclonal antibody, PBS Only

• Featured Product
• KD/KO Validated

PPP2R5A Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Conjugate

Unconjugated

Cat no : 12675-2-PBS

Synonyms

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Validation Data Gallery

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  WB analysis of HeLa using 12675-2-AP (same clone as 12675-2-PBS)

  WB result of PPP2R5A antibody (12675-2-AP; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-PPP2R5A transfected HeLa cells. This data was developed using the same antibody clone with 12675-2-PBS in a different storage buffer formulation.

• 

  WB analysis of human ileum using 12675-2-AP (same clone as 12675-2-PBS)

  human ileum tissue were subjected to SDS PAGE followed by western blot with 12675-2-AP (PPP2R5A antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12675-2-PBS in a different storage buffer formulation.

• 

  WB analysis of human heart using 12675-2-AP (same clone as 12675-2-PBS)

  human heart tissue were subjected to SDS PAGE followed by western blot with 12675-2-AP (PPP2R5A antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12675-2-PBS in a different storage buffer formulation.

• 

  WB analysis using 12675-2-AP (same clone as 12675-2-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 12675-2-AP (PPP2R5A antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12675-2-PBS in a different storage buffer formulation.

• 

  WB analysis of HeLa using 12675-2-AP (same clone as 12675-2-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 12675-2-AP (PPP2R5A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12675-2-PBS in a different storage buffer formulation.

• 

  WB analysis of human kidney using 12675-2-AP (same clone as 12675-2-PBS)

  human kidney tissue were subjected to SDS PAGE followed by western blot with 12675-2-AP (PPP2R5A antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12675-2-PBS in a different storage buffer formulation.

• 

  WB analysis of human lung using 12675-2-AP (same clone as 12675-2-PBS)

  human lung tissue were subjected to SDS PAGE followed by western blot with 12675-2-AP (PPP2R5A antibody) at dilution of 1:0 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12675-2-PBS in a different storage buffer formulation.

• 

  WB analysis of human brain using 12675-2-AP (same clone as 12675-2-PBS)

  human brain tissue were subjected to SDS PAGE followed by western blot with 12675-2-AP (PPP2R5A antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12675-2-PBS in a different storage buffer formulation.

• 

  IP experiment of mouse brain using 12675-2-AP (same clone as 12675-2-PBS)

  IP result of anti-PPP2R5A (IP:12675-2-AP, 4ug; Detection:12675-2-AP 1:500) with mouse brain tissue lysate 4000ug. This data was developed using the same antibody clone with 12675-2-PBS in a different storage buffer formulation.

• 

  IHC staining of human pancreas cancer using 12675-2-AP (same clone as 12675-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 12675-2-AP (PPP2R5A antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12675-2-PBS in a different storage buffer formulation.

• 

  IHC staining of human pancreas cancer using 12675-2-AP (same clone as 12675-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 12675-2-AP (PPP2R5A antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12675-2-PBS in a different storage buffer formulation.

• 

  IHC staining of human pancreas cancer using 12675-2-AP (same clone as 12675-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 12675-2-AP (PPP2R5A antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12675-2-PBS in a different storage buffer formulation.

• 

  IF Staining of HepG2 using 12675-2-AP (same clone as 12675-2-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 12675-2-AP (PPP2R5A antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 12675-2-PBS in a different storage buffer formulation.

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Product Information

12675-2-PBS targets PPP2R5A in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PPP2R5A fusion protein Ag3360
• Full Name: protein phosphatase 2, regulatory subunit B', alpha isoform
• Calculated Molecular Weight: 486 aa, 56 kDa
• Observed Molecular Weight: 56 kDa
• GenBank Accession Number: BC022474
• Gene Symbol: PPP2R5A
• Gene ID (NCBI): 5525
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

PPP2R5A(protein phosphatase 2, regulatory subunit B (B56), alpha isoform) is also named as PR61alpha and belongs to the phosphatase 2A regulatory subunit B56 family. B56 alpha subunit gene encodes a cytoplasmic phosphoprotein(PMID:7592815). The B regulatory subunit might modulate substrate selectivity and catalytic activity, and also might direct the localization of the catalytic enzyme to a particular subcellular compartment. This protein has 2 isoforms produced by alternative splicing with the molecular weight of 54 kDa and 50 kDa.