PPP2R3C Polyclonal antibody

PPP2R3C Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 24877-1-AP

Synonyms

Serine/threonine-protein phosphatase 2A regulatory subunit B'' subunit gamma, Rhabdomyosarcoma antigen MU-RMS-40.6A/6C, G5PR, G4 1, C14orf10

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Validation Data Gallery

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  WB analysis of mouse brain using 24877-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 24877-1-AP (PPP2R3C Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat brain using 24877-1-AP

  rat brain tissue were subjected to SDS PAGE followed by western blot with 24877-1-AP (PPP2R3C Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human brain using 24877-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 24877-1-AP (PPP2R3C Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human brain using 24877-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 24877-1-AP (PPP2R3C Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human stomach cancer using 24877-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 24877-1-AP (PPP2R3C Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human stomach cancer using 24877-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 24877-1-AP (PPP2R3C Antibody) at dilution of 1:50 (under 40x lens).

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Tested Applications

• Positive WB detected in: mouse brain tissue, rat brain tissue
• Positive IHC detected in: human brain tissue, human stomach cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

24877-1-AP targets PPP2R3C in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PPP2R3C fusion protein Ag19282
• Full Name: protein phosphatase 2 (formerly 2A), regulatory subunit B'', gamma
• Calculated Molecular Weight: 453 aa, 53 kDa
• Observed Molecular Weight: 53 kDa
• GenBank Accession Number: BC063438
• Gene Symbol: PPP2R3C
• Gene ID (NCBI): 55012
• RRID: AB_2879773
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PPP2R3C(Serine/threonine-protein phosphatase 2A regulatory subunit B'' subunit gamma) is also named as C14orf10, and G5PR. It may regulate MCM3AP phosphorylation through phosphatase recruitment and play a role in the activation-induced cell death of B-cells. It has 2 isoforms produced by alternative splicing with the molecular weight of 53 kDa and 40 kDa.