PPP2R1B Polyclonal antibody

• Featured Product
• KD/KO Validated

PPP2R1B Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, CoIP, ELISA

Conjugate

Unconjugated

Publications

3

Cat no : 12621-1-AP

Synonyms

Serine/threonine-protein phosphatase 2A 65 kDa regulatory subunit A beta isoform, PR65B, PP2A subunit A isoform R1-beta, PP2A subunit A isoform R1 beta, PP2A subunit A isoform PR65-beta

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Validation Data Gallery

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  WB analysis using 12621-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12621-1-AP (PPP2R1B antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis using 12621-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12621-1-AP (PPP2R1B antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of human kidney using 12621-1-AP

  human kidney tissue were subjected to SDS PAGE followed by western blot with 12621-1-AP (PPP2R1B antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver using 12621-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 12621-1-AP (PPP2R1B antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver using 12621-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 12621-1-AP (PPP2R1B antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of Caco-2 using 12621-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed Caco-2 cells using PPP2R1B antibody (12621-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: A2780 cells, human kidney tissue, mouse testis tissue, rat testis tissue, HeLa cells
• Positive IHC detected in: human liver tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: Caco-2 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 3 publications below
• IHC: See 1 publications below
• CoIP: See 1 publications below

Product Information

12621-1-AP targets PPP2R1B in WB, IHC, IF/ICC, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PPP2R1B fusion protein Ag3307
• Full Name: protein phosphatase 2 (formerly 2A), regulatory subunit A, beta isoform
• Calculated Molecular Weight: 667 aa, 74 kDa
• Observed Molecular Weight: 60-74 kDa
• GenBank Accession Number: BC027596
• Gene Symbol: PPP2R1B
• Gene ID (NCBI): 5519
• RRID: AB_2169941
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB	Front Cell Dev Biol; BRG1 Activates PR65A Transcription to Regulate NO Bioavailability in Vascular Endothelial Cells.; Authors - Baoyu Chen
mouse	WB	EMBO Rep; CIP2A deficiency promotes depression-like behaviors in mice through inhibition of dendritic arborization; Authors - Wen-Ting Hu
human,mouse	WB,IHC,CoIP	Cancer Cell Int; PPP2R1B abolishes colorectal cancer liver metastasis and sensitizes Oxaliplatin by inhibiting MAPK/ERK signaling pathway; Authors - Wei Liu ; KD Validated