Neurabin 2 Polyclonal antibody

• Featured Product
• KD/KO Validated

Neurabin 2 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Publications

4

Cat no : 55129-1-AP

Synonyms

Neurabin 2, Neurabin II, PPP1R6, PPP1R9, PPP1R9B, SPINO, Spinophilin, Spn

---

Validation Data Gallery

• 

  WB analysis of mouse brain using 55129-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 55129-1-AP (Neurabin 2 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A549 using 55129-1-AP

  WB result of PPP1R9B antibody (55129-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Neurabin 2 transfected A549 cells.

• 

  IP experiment of mouse brain using 55129-1-AP

  IP result of anti-Neurabin 2 (IP:55129-1-AP, 4ug; Detection:55129-1-AP 1:300) with mouse brain tissue lysate 6000ug.

• 

  IHC staining of human brain using 55129-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 55129-1-AP (Neurabin 2 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human brain using 55129-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 55129-1-AP (Neurabin 2 antibody) at dilution of 1:50 (under 40x lens).

---

Tested Applications

• Positive WB detected in: mouse brain tissue, A549 cells
• Positive IP detected in: mouse brain tissue
• Positive IHC detected in: human brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:8000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 4 publications below
• IP: See 1 publications below

Product Information

55129-1-AP targets Neurabin 2 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: protein phosphatase 1, regulatory (inhibitor) subunit 9B
• Calculated Molecular Weight: 89 kDa
• Observed Molecular Weight: 120-130 kDa
• GenBank Accession Number: NM_032595
• Gene Symbol: Spinophilin/Neurabin 2
• Gene ID (NCBI): 84687
• RRID: AB_10837358
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: 0.1M NaHCO3, 0.1M glycine, 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Neurabin 2, also named as Spinophilin, seems to act as a scaffold protein in multiple signaling pathways. It modulates excitatory synaptic transmission and dendritic spine morphology. PPP1R9B binds to actin filaments (F-actin) and shows cross-linking activity. It may play an important role in linking the actin cytoskeleton to the plasma membrane at the synaptic junction. PPP1R9B plays a role in regulation of G-protein coupled receptor signaling, including D2Rs and alpha-adrenergic receptors. PPP1R9B probably regulates p70 S6 kinase activity by forming a complex with TIAM. The antibody is specific to PPP1R9B. The predicted molecular weight of spinophilin is 89 kDa, which differs significantly from the apparent MW seen in SDS/PAGE. Both the expressed full-length cDNA and the endogenous protein run at 120-130 kDa. This may be due to an extended conformation and/or low SDS binding capacity. (PMID: 28941770, PMID: 9275233).

Publications

Species	Application	Title
human	WB,IP	Cell Death Differ; Lysine methylation of PPP1CA by the methyltransferase SUV39H2 disrupts TFEB-dependent autophagy and promotes intervertebral disc degeneration; Authors - Huaizhen Liang
mouse	WB	Neural Regen Res; 3'-Deoxyadenosin alleviates methamphetamine-induced aberrant synaptic plasticity and seeking behavior by inhibiting the NLRP3 inflammasome; Authors - Yize Qi
	WB	Toxicology; Changes in memory and synaptic plasticity induced in male rats after maternal exposure to Bisphenol A.; Authors - Chong Wang
mouse	WB	Genes Dis; Hippocampal overexpression of TREM2 ameliorates high fat diet induced cognitive impairment and modulates phenotypic polarization of the microglia.; Authors - Min Wu